Enhanced elevations of hypo-osmotic shock-induced cytosolic and nucleic calcium concentrations in tobacco cells by pretreatment with dimethyl sulfoxide
| Autor: | Hieu T H Nguyen, Masaki Kuse, Tomonori Kawano, François Bouteau, Christian Mazars |
|---|---|
| Přispěvatelé: | Duy Tan University (DTU), Université Sorbonne Paris Cité (USPC), Laboratoire de Recherche en Sciences Végétales (LRSV), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Interactions Arbres-Microorganismes (IAM), Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Luminescence Osmotic shock Transgene Aequorin chemistry.chemical_element Calcium 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Cytosol Osmotic Pressure Tobacco [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Dimethyl Sulfoxide Molecular Biology ComputingMilieux_MISCELLANEOUS Cell Nucleus biology Chemistry Dimethyl sulfoxide Organic Chemistry General Medicine Plants Genetically Modified Cell Compartmentation 3. Good health Solvent 030104 developmental biology biology.protein Nucleic acid Biophysics 010606 plant biology & botany Biotechnology |
| Zdroj: | Bioscience, Biotechnology and Biochemistry Bioscience, Biotechnology and Biochemistry, Taylor & Francis, 2018, 83 (2), pp.318-321. ⟨10.1080/09168451.2018.1533801⟩ |
| ISSN: | 0916-8451 1347-6947 |
| Popis: | Dimethyl sulfoxide (DMSO) is a dipolar aprotic solvent widely used in biological assays. Here, we observed that DMSO enhanced the hypo-osmotically induced increases in the concentration of Ca2+ in cytosolic and nucleic compartments in the transgenic cell-lines of tobacco (BY-2) expressing aequorin. |
| Databáze: | OpenAIRE |
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