Impact of beta 2-microglobulin on tapasin expression and covalent association
| Autor: | Joyce C. Solheim, Brittney L. Smith, Laura C. Simone |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Cellular immunity
Immunology Antigen presentation Blotting Western Protein Disulfide-Isomerases Gene Expression Major histocompatibility complex Article Mice Tapasin Cell Line Tumor MHC class I Cytotoxic T cell Animals Humans Disulfides biology Beta-2 microglobulin Reverse Transcriptase Polymerase Chain Reaction Histocompatibility Antigens Class I Membrane Transport Proteins Transporter associated with antigen processing Molecular biology Cell biology biology.protein beta 2-Microglobulin Protein Binding |
| Popis: | Cellular immunity is dependent on major histocompatibility complex (MHC) class I molecules enabling cytotoxic T cell recognition of malignant and infected cells. Loading of antigenic peptides onto MHC class I is assisted by a peptide-loading protein complex including tapasin. We found that tapasin expression is enhanced by beta 2-microglobulin via both transcriptional and post-transcriptional mechanisms. In addition, using conditions which preserve the tapasin-ERp57 disulfide-bonded conjugate, we demonstrated that beta 2-microglobulin increases tapasin-containing protein complexes, and reduces the level of MHC class I/ERp57 complexes lacking tapasin. Overall, our results provide a new perspective on the regulation of tapasin expression and association. |
| Databáze: | OpenAIRE |
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