Lamina Cribrosa Microarchitecture in Monkey Early Experimental Glaucoma: Global Change
| Autor: | Howard Lockwood, Galen Williams, Hongli Yang, Stuart K. Gardiner, Claude F. Burgoyne, Juan Reynaud |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Lamina Intraocular pressure Pore diameter Materials science genetic structures Optic Disk Optic disk Connective tissue Glaucoma Cell Count 03 medical and health sciences 0302 clinical medicine Imaging Three-Dimensional Optic Nerve Diseases medicine Image Processing Computer-Assisted Animals Optic Nerve Heads Intraocular Pressure business.industry optic nerve head Axial length Anatomy medicine.disease Macaca mulatta eye diseases Axons Axial Length Eye Disease Models Animal 030104 developmental biology medicine.anatomical_structure Connective Tissue 030221 ophthalmology & optometry sense organs Nuclear medicine business lamina cribrosa |
| Zdroj: | Investigative Ophthalmology & Visual Science |
| ISSN: | 1552-5783 |
| Popis: | PURPOSE The purpose of this study was to characterize experimental glaucoma (EG) versus control eye differences in lamina cribrosa (LC), beam diameter (BD), pore diameter (PD), connective tissue volume fraction (CTVF), connective tissue volume (CTV), and LC volume (LV) in monkey early EG. METHODS Optic nerve heads (ONHs) of 14 unilateral EG and 6 bilateral normal (BN) monkeys underwent three-dimensional reconstruction and LC beam segmentation. Each beam and pore voxel was assigned a diameter based on the largest sphere that contained it before transformation to a common cylinder with inner, middle, and outer layers. Full-thickness and layer averages for BD, PD, CTVF, CTV, and LV were calculated for each ONH. Beam diameter and PD distributions for each ONH were fit to a gamma distribution and summarized by scale and shape parameters. Experimental glaucoma and depth effects were assessed for each parameter by linear mixed-effects (LME) modeling. Animal-specific EG versus control eye differences that exceeded the maximum intereye difference among the six BN animals were considered significant. RESULTS Overall EG eye mean PD was 12.8% larger (28.2 ± 5.6 vs. 25.0 ± 3.3 μm), CTV was 26.5% larger (100.06 ± 47.98 vs. 79.12 ± 28.35 × 106 μm3), and LV was 40% larger (229.29 ± 98.19 vs. 163.63 ± 39.87 × 106 μm3) than control eyes (P ≤ 0.05, LME). Experimental glaucoma effects were significantly different by layer for PD (P = 0.0097) and CTVF (P < 0.0001). Pore diameter expanded consistently across all PDs. Experimental glaucoma eye-specific parameter change was variable in magnitude and direction. CONCLUSIONS Pore diameter, CTV, and LV increase in monkey early EG; however, EG eye-specific change is variable and includes both increases and decreases in BD and CTVF. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|