LDB1 and the SWI/SNF complex participate in both transcriptional activation and repression by Caenorhabditis elegans BLIMP1/PRDM1
| Autor: | Hei Tung Fong, Thilo Hagen, Takao Inoue |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Transcriptional Activation
Chromosomal Proteins Non-Histone Protein subunit Biophysics Biochemistry 03 medical and health sciences 0302 clinical medicine Genes Reporter Structural Biology Cell Line Tumor hemic and lymphatic diseases Gene expression Genetics Transcriptional regulation Animals Humans Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Transcription factor Psychological repression 030304 developmental biology Regulation of gene expression 0303 health sciences biology SWI/SNF complex LIM Domain Proteins biology.organism_classification Cell biology DNA-Binding Proteins Enhancer Elements Genetic Gene Expression Regulation RNA Interference Positive Regulatory Domain I-Binding Factor 1 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. 1863:194577 |
| ISSN: | 1874-9399 |
| DOI: | 10.1016/j.bbagrm.2020.194577 |
| Popis: | Transcription factors of the BLIMP1/PRDM1 family are important regulators of development. BLIMP1/PRDM1 can both activate and repress gene expression, however, the mechanism of activation is not well understood. Therefore, we looked for factors involved in gene activation by C. elegans BLMP-1, the ortholog of BLIMP1/PRDM1. BLMP-1 activates the expression of bed-3, a gene involved in vulval development. By screening nuclear proteins that function in vulval development, we identified two proteins (LDB-1 and HAM-3) required for BLMP-1 dependent bed-3 expression. LDB-1 is the sole C. elegans member of the LIM Binding Protein (LDB) family, whereas HAM-3 is an accessory subunit of the SWI/SNF complex (ortholog of human SMARCD3/BAF60C). A core SWI/SNF subunit SWSN-1 (ortholog of human SMARCC1/BAF155) is also involved. We found that LDB-1 and HAM-3 bind to BLMP-1, suggesting that BLMP-1 recruits LDB-1 and the SWI/SNF complex to activate bed-3 expression. Interestingly, LDB-1 and HAM-3 are involved in both transcriptional activation and repression. In particular, BLMP-1, LDB-1 and HAM-3 co-regulate a set of hypodermal genes including bed-3 (activated), col-124 (activated) and lin-29 (repressed). On the other hand, LDB-1 and HAM-3 are not required for activation or repression of some genes regulated by BLMP-1 (e.g. T09D3.8, nas-10). We also found that human LDB1, SMARCD3/BAF60C and SMARCC1/BAF155 all physically interact with human BLIMP1/PRDM1 in vitro and are closely associated with BLIMP1/PRDM1 in vivo. Taken together, these results identify LDB1 and SWI/SNF as likely conserved cofactors of BLIMP1/PRDM1, which participate in activation and repression of a subset of BLIMP1/PRDM1-regulated genes. |
| Databáze: | OpenAIRE |
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