The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen
| Autor: | Alan J. Davidson, Veronika Sander, Shantanu Patke, Jung Y Lee, Young-Tae Chang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Boron Compounds
0301 basic medicine CDr10b vital dye Retinoic acid Pharmaceutical Science Lumen (anatomy) Tretinoin Biology Article Analytical Chemistry lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Organic chemistry Drug Discovery medicine Zebrafish larvae Animals Physical and Theoretical Chemistry Zebrafish Developmental Malformations Fluorescent reporter Organic Chemistry zebrafish gut development mid-intestine laser ablation biology.organism_classification Small intestine Epithelium Cell biology Intestines 030104 developmental biology medicine.anatomical_structure Microscopy Fluorescence Biochemistry chemistry Chemistry (miscellaneous) Larva Molecular Medicine 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules, Vol 22, Iss 3, p 454 (2017) Molecules; Volume 22; Issue 3; Pages: 454 |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules22030454 |
| Popis: | We describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small intestine. By perturbing retinoic acid signaling, which regulates the size of the mid-intestine segment, we show that CDr10b is a valuable tool to rapidly assess developmental malformations of the intestine in live animals. |
| Databáze: | OpenAIRE |
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