Chromosomal abnormalities in Day-6, in vitro-produced pig embryos
| Autor: | Tod C. McCauley, M.R. Mazza, Jiude Mao, D. Di Berardino, Brad A. Didion, Guangming Wu, Gianfranco Coppola, Giuseppe Coppola, Billy N. Day |
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| Přispěvatelé: | Mccauley, T, Mazza, Mr, Didion, Ba, Mao, J, Wu, G, Coppola, G, DI BERARDINO, Dino, Day, Bn |
| Rok vydání: | 2003 |
| Předmět: |
animal structures
Mitotic index Swine Cell Count Fertilization in Vitro Biology Chromosome Andrology Embryonic and Fetal Development Human fertilization Abnormalitie Food Animals Polyploid Culture Techniques Animals Small Animals Chromosome Aberrations Genetics Pig embryo Ploidies Equine Polyspermy Karyotype Embryo Embryo Mammalian Karyotyping embryonic structures Animal Science and Zoology Ploidy |
| Zdroj: | Theriogenology. 60:1569-1580 |
| ISSN: | 0093-691X |
| Popis: | A cytogenetic study was undertaken to quantify, by chromosomal karyotyping, the incidence and type of chromosomal abnormalities present in Day-6 in vitro-produced (IVP) porcine embryos. Morphologically normal Day-6 blastocysts (n=318) were fixed and grouped into six classes according to the number of total cells (fromor =20 to 61-70). Of 248 embryos suitable for analysis, 97 (39.1%) displayed chromosomal abnormalities. The abnormalities included haploidy (9.3%), polyploidy (71.1%) and mixoploidy (19.6%). Within polyploid embryos, triploidy and tetraploidy showed the highest incidence (56.5 and 27.5%, respectively); among mixoploid embryos, diploid-triploid embryos (2n/3n) were prevalent (36.8%). Overall, the mean cell number was 34.3 +/- 12.1 and the mitotic index was 8.6 +/- 6.1. Chromosomally abnormal embryos had fewer (P0.01) total cells compared to normal (2n) embryos (31.8 +/- 1.3 versus 35.9 +/- 1.0). In addition, the incidence of polyploidy decreased as the number of cells increased, while that of mixoploidy did not differ. These data indicate that polyploidy affects a large percentage of IVP porcine embryos capable of developing to blastocysts and the incidence of chromosomal abnormalities is much higher than that reported previously in in vivo embryos in this species. Given the ability of morphologically normal embryos with an abnormal chromosome complement to undergo preimplantation development in vitro, and the inability to identify blastocysts with abnormal karyotype without cytogenetic analysis, careful consideration should be given to factors affecting ploidy of IVP embryos, especially the incidence of polyspermic fertilization, when evaluating criteria of a porcine in vitro embryo production scheme. |
| Databáze: | OpenAIRE |
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