Kinetic fingerprinting links bacteria-phage interactions with emergent dynamics: Rapid depletion of klebsiella pneumoniae indicates phage synergy
| Autor: | Marie Anders-Maurer, Johannes Wittmann, Oleg Krut, Christine Rohde, Insea Schlattmeier, Holger Loessner, Christel Kamp, Cornelia Pokalyuk, Isabelle Bekeredjian-Ding |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) phage therapy kinetic fingerprint Phage therapy Klebsiella pneumoniae medicine.medical_treatment viruses 030106 microbiology Population Bacterial population High multiplicity Biochemistry Microbiology resistance 03 medical and health sciences medicine population dynamics phage emergence Pharmacology (medical) ddc:610 General Pharmacology Toxicology and Pharmaceutics education education.field_of_study biology Chemistry Dynamics (mechanics) lcsh:RM1-950 Rational design mathematical modeling biology.organism_classification 030104 developmental biology Infectious Diseases lcsh:Therapeutics. Pharmacology synergistic interaction Phagentherapie Bacteria |
| Zdroj: | Antibiotics, Vol 9, Iss 408, p 408 (2020) Antibiotics, 9(7):408 Antibiotics Volume 9 Issue 7 |
| Popis: | The specific temporal evolution of bacterial and phage population sizes, in particular bacterial depletion and the emergence of a resistant bacterial population, can be seen as a kinetic fingerprint that depends on the manifold interactions of the specific phage&ndash host pair during the course of infection. We have elaborated such a kinetic fingerprint for a human urinary tract Klebsiella pneumoniae isolate and its phage vB_KpnP_Lessing by a modeling approach based on data from in vitro co-culture. We found a faster depletion of the initially sensitive bacterial population than expected from simple mass action kinetics. A possible explanation for the rapid decline of the bacterial population is a synergistic interaction of phages which can be a favorable feature for phage therapies. In addition to this interaction characteristic, analysis of the kinetic fingerprint of this bacteria and phage combination revealed several relevant aspects of their population dynamics: A reduction of the bacterial concentration can be achieved only at high multiplicity of infection whereas bacterial extinction is hardly accomplished. Furthermore the binding affinity of the phage to bacteria is identified as one of the most crucial parameters for the reduction of the bacterial population size. Thus, kinetic fingerprinting can be used to infer phage&ndash host interactions and to explore emergent dynamics which facilitates a rational design of phage therapies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|