Line scanning, fiber bundle fluorescence HiLo endomicroscopy with confocal slit detection
| Autor: | Guang-Zhong Yang, Khushi Vyas, Haojie Zhang |
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| Přispěvatelé: | Engineering & Physical Science Research Council (EPSRC) |
| Rok vydání: | 2019 |
| Předmět: |
Paper
Materials science Light Optical sectioning Swine Confocal 0205 Optical Physics Biomedical Engineering Kidney 01 natural sciences law.invention 010309 optics Biomaterials 03 medical and health sciences 0903 Biomedical Engineering Confocal microscopy law 0103 physical sciences Image Processing Computer-Assisted Fluorescence microscope Endomicroscopy Animals Fiber Optic Technology Optical Fibers 030304 developmental biology optical microscopy Microscopy 0303 health sciences CMOS sensor Microscopy Confocal Endoscopy Optics Equipment Design Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials 1113 Opthalmology and Optometry Lens (optics) fluorescence spatial light modulators biomedical imaging Preclinical imaging Biomedical engineering |
| Zdroj: | Journal of Biomedical Optics |
| Popis: | Fiber bundle fluorescence endomicroscopy is an effective method for in vivo imaging of biological tissue samples. Line-scanning confocal laser endomicroscopy realizes confocal imaging at a much higher frame rate compared to the point scanning system, but with reduced optical sectioning. To address this problem, we describe a fiber bundle endomicroscopy system that utilizes the HiLo technique to enhance the optical sectioning while still maintaining high image acquisition rates. Confocal HiLo endomicroscopy is achieved by synchronizing the scanning hybrid-illumination laser line with the rolling shutter of a CMOS camera. An evident improvement of axial sectioning is achieved as compared to the line-scanning confocal endomicroscopy without the HiLo technique. Comparisons are also made with epifluorescence endomicroscopy with and without HiLo. The optical sectioning enhancement is demonstrated on lens tissue as well as porcine kidney tissue. |
| Databáze: | OpenAIRE |
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