SENSITIVITY OF EARLY EMBRYONIC THYMIC LYMPHOID DEVELOPMENT TO3H-THYMIDINE OF HIGH SPECIFIC ACTIVITY
| Autor: | Gunnar V. Alm, Roger Juhlin |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
medicine.medical_specialty
Time Factors animal structures Cell Survival Extraembryonic Membranes Cell Count Chick Embryo Thymus Gland Biology Tritium Organ culture chemistry.chemical_compound Thymic epithelium Organ Culture Techniques Internal medicine Precursor cell medicine Animals Transplantation Homologous Lymphocytes Age Factors Embryo Cell Biology General Medicine Embryonic stem cell Cell biology Radiation Effects Chorioallantoic membrane Endocrinology chemistry High specific activity embryonic structures Thymidine Cell Division |
| Zdroj: | Cell Proliferation. 7:327-336 |
| ISSN: | 1365-2184 0960-7722 |
| DOI: | 10.1111/j.1365-2184.1974.tb00415.x |
| Popis: | The pulse technique, using high specific activity 3H-TdR to selectively kill cells in cell cycle, was applied to the thymic anlagen of chick embryos. With optimal specific and total 3H-TdR activities and pulse times of 2–4 hr the subsequent lymphoid development in organ culture of the thymic anlagen of 10-day-old chick embryos could be almost completely inhibited. The most important effect of the 3H-TdR was on the lymphoid precursor cells of the anlagen. The thymic epithelium appeared more resistant to 3H-TdR and allowed a lymphoid development of pulsed anlagen grafted to the chorioallantoic membrane of chick embryos when new lymphoid precursor cells were provided. The lymphoid precursor cells of the thymic anlagen of 10-day-old chick embryos therefore appeared to be in cell cycle with short generation time. The thymic anlagen of 8-, 9- and 10-day-old but not 7-day-old embryos showed a lymphoid development in organ culture. They did not differ with respect to the sensitivity to hot pulses of 3H-TdR. Thus no evidence of a lag in the onset of lymphoid precursor cell proliferation during the development of the early embryonic chick thymus was noted. |
| Databáze: | OpenAIRE |
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