Zebrafish Embryo Infection Model to Investigate Pseudomonas aeruginosa Interaction With Innate Immunity and Validate New Therapeutics
Autor: | Anne-Béatrice Blanc-Potard, Stéphane Pont |
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Přispěvatelé: | LPHI - Laboratory of Pathogen Host Interactions (LPHI), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) |
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Microbiology (medical)
Cystic Fibrosis [SDV]Life Sciences [q-bio] Immunology Virulence Human pathogen Context (language use) Review Biology medicine.disease_cause Microbiology Cystic fibrosis 03 medical and health sciences Cellular and Infection Microbiology medicine Animals Humans Pseudomonas Infections drug screening innate immunity Pathogen Zebrafish 030304 developmental biology 0303 health sciences Innate immune system 030306 microbiology Pseudomonas aeruginosa medicine.disease biology.organism_classification QR1-502 Immunity Innate 3. Good health Infectious Diseases host–pathogen interactions |
Zdroj: | Frontiers in Cellular and Infection Microbiology Frontiers in Cellular and Infection Microbiology, Frontiers, 2021, 11, ⟨10.3389/fcimb.2021.745851⟩ Frontiers in Cellular and Infection Microbiology, Vol 11 (2021) |
ISSN: | 2235-2988 |
Popis: | The opportunistic human pathogen Pseudomonas aeruginosa is responsible for a variety of acute infections and is a major cause of mortality in chronically infected patients with cystic fibrosis (CF). Considering the intrinsic and acquired resistance of P. aeruginosa to currently used antibiotics, new therapeutic strategies against this pathogen are urgently needed. Whereas virulence factors of P. aeruginosa are well characterized, the interplay between P. aeruginosa and the innate immune response during infection remains unclear. Zebrafish embryo is now firmly established as a potent vertebrate model for the study of infectious human diseases, due to strong similarities of its innate immune system with that of humans and the unprecedented possibilities of non-invasive real-time imaging. This model has been successfully developed to investigate the contribution of bacterial and host factors involved in P. aeruginosa pathogenesis, as well as rapidly assess the efficacy of anti-Pseudomonas molecules. Importantly, zebrafish embryo appears as the state-of-the-art model to address in vivo the contribution of innate immunity in the outcome of P. aeruginosa infection. Of interest, is the finding that the zebrafish encodes a CFTR channel closely related to human CFTR, which allowed to develop a model to address P. aeruginosa pathogenesis, innate immune response, and treatment evaluation in a CF context. |
Databáze: | OpenAIRE |
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