3D-Printed Biodegradable Microswimmer for Theranostic Cargo Delivery and Release
| Autor: | Metin Sitti, Immihan Ceren Yasa, Hakan Ceylan, Ahmet Fatih Tabak, Oncay Yasa, Joshua Giltinan |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
3d printed
food.ingredient Materials science Superparamagnetic iron oxide nanoparticles Cell Survival Surface Properties General Physics and Astronomy Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Gelatin biodegradation Ferric Compounds Article Theranostic Nanomedicine Polymerization gelatin food Drug Delivery Systems Cell Line Tumor Humans General Materials Science Particle Size Magnetite Nanoparticles Photons General Engineering targeted delivery Anticoagulants Dextrans 3D printing 021001 nanoscience & nanotechnology 0104 chemical sciences Drug Liberation Magnetic Fields Drug delivery drug delivery Printing Three-Dimensional Environmental sensing Matrix Metalloproteinase 2 Methacrylates hydrogel 0210 nano-technology microrobot |
| Zdroj: | ACS Nano |
| ISSN: | 1936-086X 1936-0851 |
| Popis: | Untethered mobile microrobots have the potential to leverage minimally invasive theranostic functions precisely and efficiently in hard-to-reach, confined, and delicate inner body sites. However, such a complex task requires an integrated design and engineering, where powering, control, environmental sensing, medical functionality, and biodegradability need to be considered altogether. The present study reports a hydrogel-based, magnetically powered and controlled, enzymatically degradable microswimmer, which is responsive to the pathological markers in its microenvironment for theranostic cargo delivery and release tasks. We design a double-helical architecture enabling volumetric cargo loading and swimming capabilities under rotational magnetic fields and a 3D-printed optimized 3D microswimmer (length = 20 μm and diameter = 6 μm) using two-photon polymerization from a magnetic precursor suspension composed from gelatin methacryloyl and biofunctionalized superparamagnetic iron oxide nanoparticles. At normal physiological concentrations, we show that matrix metalloproteinase-2 (MMP-2) enzyme could entirely degrade the microswimmer in 118 h to solubilized nontoxic products. The microswimmer rapidly responds to the pathological concentrations of MMP-2 by swelling and thereby boosting the release of the embedded cargo molecules. In addition to delivery of the drug type of therapeutic cargo molecules completely to the given microenvironment after full degradation, microswimmers can also release other functional cargos. As an example demonstration, anti-ErbB 2 antibody-tagged magnetic nanoparticles are released from the fully degraded microswimmers for targeted labeling of SKBR3 breast cancer cells in vitro toward a potential future scenario of medical imaging of remaining cancer tissue sites after a microswimmer-based therapeutic delivery operation. |
| Databáze: | OpenAIRE |
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