Distinct Subsets of CD1d-restricted T Cells Recognize Self-antigens Loaded in Different Cellular Compartments
| Autor: | Ya Hui Chiu, Alice Dautry-Varsat, Albert Bendelac, Daniel Lee, Jayanthi Jayawardena, Se Ho Park, Angela Weiss |
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| Rok vydání: | 1999 |
| Předmět: |
self-antigen
CD3 Complex T-Lymphocytes T cell Immunology Receptors Antigen T-Cell CD1 Autoimmunity Mice Transgenic chemical and pharmacologic phenomena Endosomes Biology interleukin 4 Antigens CD1 Interferon-gamma Mice Interleukin 21 medicine Animals Immunology and Allergy Cytotoxic T cell IL-2 receptor Antigens Antigen-presenting cell endosome interferon γ Hybridomas ZAP70 hemic and immune systems Articles Receptors Neurokinin-1 Natural killer T cell Molecular biology medicine.anatomical_structure CD4 Antigens Interleukin-4 |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| DOI: | 10.1084/jem.189.1.103 |
| Popis: | Although recent studies have indicated that the major histocompatibility complex-like, beta2-microglobulin-associated CD1 molecules might function to present a novel chemical class of antigens, lipids and glycolipids, to alpha/beta T cells, little is known about the T cell subsets that interact with CD1. A subset of CD1d-autoreactive, natural killer (NK)1.1 receptor-expressing alpha/beta T cells has recently been identified. These cells, which include both CD4(-)CD8(-) and CD4(+) T cells, preferentially use an invariant Valpha14-Jalpha281 T cell receptor (TCR) alpha chain paired with a Vbeta8 TCR beta chain in mice, or the homologous Valpha24-JalphaQ/Vbeta11 in humans. This cell subset can explosively release key cytokines such as interleukin (IL)-4 and interferon (IFN)-gamma upon TCR engagement and may regulate a variety of infectious and autoimmune conditions. Here, we report the existence of a second subset of CD1d-restricted CD4(+) T cells that do not express the NK1.1 receptor or the Valpha14 TCR. Like the Valpha14(+) NK1.1(+) T cells, these T cells exhibit a high frequency of autoreactivity to CD1d, use a restricted albeit distinct set of TCR gene families, and contribute to the early burst of IL-4 and IFN-gamma induced by intravenous injection of anti-CD3. However, the Valpha14(+) NK1.1(+) and Valpha14(-) NK1.1(-) T cells differ markedly in their requirements for self-antigen presentation. Antigen presentation to the Valpha14(+) NK1.1(+) cells requires endosomal targeting of CD1d through a tail-encoded tyrosine-based motif, whereas antigen presentation to the Valpha14(-) NK1.1(-) cells does not. These experiments suggest the existence of two phenotypically different subsets of CD1d-restricted T cells that survey self-antigens loaded in distinct cellular compartments. |
| Databáze: | OpenAIRE |
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