Reversal of persistent fibrosis in aging by targeting Nox4-Nrf2 redox imbalance
| Autor: | Ashish Kurundkar, Thomas D. Hock, Louise Hecker, Eric Meldrum, Victor J. Thannickal, Naomi J. Logsdon, Deepali Kurundkar, Yan Y. Sanders, Karen Bernard |
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| Rok vydání: | 2014 |
| Předmět: |
Senescence
Aging NF-E2-Related Factor 2 Apoptosis Biology Lung injury medicine.disease_cause Models Biological Antioxidants Idiopathic pulmonary fibrosis Fibrosis medicine Animals Humans Molecular Targeted Therapy Lung NOX4 NADPH Oxidases General Medicine Lung Injury respiratory system Fibroblasts medicine.disease Idiopathic Pulmonary Fibrosis Mice Inbred C57BL Oxidative Stress medicine.anatomical_structure Phenotype NADPH Oxidase 4 Immunology Female Myofibroblast Oxidation-Reduction Oxidative stress |
| Zdroj: | Science translational medicine. 6(231) |
| ISSN: | 1946-6242 |
| Popis: | The incidence and prevalence of pathological fibrosis increase with advancing age, although mechanisms for this association are unclear. We assessed the capacity for repair of lung injury in young (2 months) and aged (18 months) mice. Whereas the severity of fibrosis was not different between these groups, aged mice demonstrated an impaired capacity for fibrosis resolution. Persistent fibrosis in lungs of aged mice was characterized by the accumulation of senescent and apoptosis-resistant myofibroblasts. These cellular phenotypes were sustained by alterations in cellular redox homeostasis resulting from elevated expression of the reactive oxygen species-generating enzyme Nox4 [NADPH (reduced form of nicotinamide adenine dinucleotide phosphate) oxidase-4] and an impaired capacity to induce the Nrf2 (NFE2-related factor 2) antioxidant response. Lung tissues from human subjects with idiopathic pulmonary fibrosis (IPF), a progressive and fatal lung disease, also demonstrated this Nox4-Nrf2 imbalance. Nox4 mediated senescence and apoptosis resistance in IPF fibroblasts. Genetic and pharmacological targeting of Nox4 in aged mice with established fibrosis attenuated the senescent, antiapoptotic myofibroblast phenotype and led to a reversal of persistent fibrosis. These studies suggest that loss of cellular redox homeostasis promotes profibrotic myofibroblast phenotypes that result in persistent fibrosis associated with aging. Our studies suggest that restoration of Nox4-Nrf2 redox balance in myofibroblasts may be a therapeutic strategy in age-associated fibrotic disorders, potentially able to resolve persistent fibrosis or even reverse its progression. |
| Databáze: | OpenAIRE |
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