Susceptibility of immortalized porcine kidney macrophages to porcine reproductive and respiratory syndrome virus-2 infection
| Autor: | Emi Wada, Takato Takenouchi, Yasutaka Nishiyama, Takeya Morozumi, Hirohide Uenishi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Swine animal diseases viruses 030106 microbiology Porcine Reproductive and Respiratory Syndrome Kidney Virus Replication Virus Cell Line 03 medical and health sciences Virology Chlorocebus aethiops Macrophages Alveolar Animals Macrophage Porcine respiratory and reproductive syndrome virus Receptor Tropism biology virus diseases respiratory system Porcine reproductive and respiratory syndrome virus biology.organism_classification Genetically modified organism 030104 developmental biology Cell culture CD163 |
| Zdroj: | C30202102010003-SC30202102110021 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2020.114026 |
| Popis: | Porcine reproductive and respiratory syndrome virus (PRRSV) displays restricted tropism to porcine alveolar macrophages in nature. Meanwhile, non-porcine cell lines derived from African green monkey kidney cell lines are permissive to PRRSV, resulting in their widespread use in PRRSV research. Furthermore, genetically modified cell lines expressing receptors targeted by PRRSV have been established. We previously established porcine immortalized kidney-derived macrophages (IPKMs) that maintained typical macrophage function. In the present study, we demonstrated the advantages of IPKMs for PRRSV research. IPKMs expressed receptors for PRRSV such as CD163 and CD169. The efficiency of virus isolation from field biological samples was higher for IPKMs than for MARC-145 cells. Five different clusters of North American type PRRSV were propagated in IPKMs. Four field strains continuously produced progeny viruses during 10 continuous passages. The efficiency of virus isolation from field biological samples and continuous progeny virus production in the sequential passages using IPKMs indicated that these cells are good vessels for PRRSV research. |
| Databáze: | OpenAIRE |
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