Progesterone receptor antagonists Org 31710 and RU 486 increase apoptosis in human periovulatory granulosa cells
| Autor: | Emilia Markström, Ruijin Shao, Eva Ch Svensson, Madeleine Andersson, Håkan Billig |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
endocrine system
medicine.medical_specialty Antagonists & inhibitors medicine.drug_class DNA Fragmentation Biology Dexamethasone GABA Antagonists Hormone Antagonists Internal medicine Follicular phase Progesterone receptor medicine Humans Picrotoxin Estrenes Ovarian follicle Furans Glucocorticoids Progesterone Electrophoresis Agar Gel Granulosa Cells Caspase 3 Obstetrics and Gynecology Dihydrotestosterone Mifepristone Receptors GABA-A Nucleosomes Spectrometry Fluorescence Endocrinology medicine.anatomical_structure Reproductive Medicine Caspases DNA fragmentation Female Gonadotropin Receptors Progesterone Luteinizing hormone medicine.drug |
| Zdroj: | Fertility and Sterility. 76:1225-1231 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/s0015-0282(01)02891-6 |
| Popis: | Objective: To investigate if progesterone receptor (PR)-mediated effects are involved in regulating the susceptibility to apoptosis in LH receptor-stimulated human luteinizing granulosa cells. Design: Laboratory study. Setting: Goteborg University and an in vitro fertilization laboratory of a university hospital. Patient(s): Women undergoing oocyte retrieval for in vitro fertilization after ovulation induction with gonadotropins. Intervention(s): Luteinizing granulosa cells were isolated from follicular aspirates after oocyte removal. The cells were treated with or without RU 486 (1 μM–100 μM), Org 31710 (1 μM–100 μM), progesterone (1 nM–10 μM), dexamethasone (0.5 μM–100 μM), dihydrotestosterone (1 nM–25 μM), RU 486 (10 μM–100 μM) + dexamethasone (50 μM), and picrotoxin (1 μM–100 μM) and were cultured under serum-free conditions. Main Outcome Measure(s): Measurement of caspase-3 activity; detection of internucleosomal DNA fragmentation using gel electrophoresis and fluorospectrophotometry; progesterone analysis of spent medium. Result(s): Addition of the PR antagonists RU 486 or Org 31710 in vitro to human luteinizing granulosa cells caused an increase in caspase-3 activity and a dose-dependent increase in internucleosomal DNA fragmentation. No effect on DNA fragmentation was seen after addition of dexamethasone, dihydrotestosterone, or picrotoxin . Conclusion(s): Nuclear PR-mediated effects are involved in regulating the susceptibility to apoptosis in LH receptor-stimulated human luteinizing granulosa cells. |
| Databáze: | OpenAIRE |
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