Touch-Spun Nanofibers for Nerve Regeneration
| Autor: | Timothy Esworthy, Raju Timsina, Sung Yun Hann, Xuan Zhou, Xiangyun Qiu, Alexander Tokarev, Lijie Grace Zhang, Darya Asheghali, Brianna Blevins, Sergiy Minko, Haitao Cui, Se-Jun Lee |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
Neurite Surface Properties Polyesters Nanofibers Biocompatible Materials macromolecular substances 02 engineering and technology 010402 general chemistry 01 natural sciences law.invention Crystallinity chemistry.chemical_compound Neural Stem Cells Tissue engineering Confocal microscopy law Animals Humans General Materials Science Fiber technology industry and agriculture Cell Differentiation musculoskeletal system 021001 nanoscience & nanotechnology Neural stem cell Nerve Regeneration 0104 chemical sciences nervous system chemistry Touch Nanofiber Polycaprolactone 0210 nano-technology Biomedical engineering |
| Zdroj: | ACS Applied Materials & Interfaces. 12:2067-2075 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.9b18614 |
| Popis: | In the current study, we examined the potential for neural stem cell (NSCs) proliferation on novel aligned touch-spun polycaprolactone (PCL) nanofibers. Electrospun PCL nanofibers with similar diameter and alignment were used as a control. Confocal microscopy images showed that NSCs grew and differentiated all over the scaffolds up to 8 days. Neurite quantification analysis revealed that the NSCs cultured on the touch-spun fibers with incorporated bovine serum albumin promoted the expression of neuron-specific class III β-tubulin after 8 days. More importantly, NSCs grown on the aligned touch-spun PCL fibers exhibited a bipolar elongation along the direction of the fiber, while NSCs cultured on the aligned electrospun PCL fibers expressed a multipolar elongation. The structural characteristics of the PCL nanofibers analyzed by X-ray diffraction indicated that the degree of crystallinity and elastic modulus of the touch-spun fiber are significantly higher than those of electrospun fibers. These findings indicate that the aligned and stiff touch-spun nanofibrous scaffolds show considerable potential for nerve injury repair. |
| Databáze: | OpenAIRE |
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