Optimization of Enzymatic Process for Vanillin Extraction Using Response Surface Methodology
| Autor: | Lehe Tan, Fenglin Gu, Zhong Chu, Wu Huasong, Fei Xu, Qing-Huang Wang |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Time Factors
Pharmaceutical Science Cellulase Article Analytical Chemistry response surface methodology lcsh:QD241-441 chemistry.chemical_compound Hydrolysis lcsh:Organic chemistry Enzymatic hydrolysis Drug Discovery Computer Graphics Response surface methodology Physical and Theoretical Chemistry extractionyield Vanilla Analysis of Variance Chromatography biology Plant Extracts Vanillin Organic Chemistry Extraction (chemistry) cellulose extraction yield Enzyme assay vanillin chemistry Chemistry (miscellaneous) Benzaldehydes Data Interpretation Statistical Yield (chemistry) Seeds extraction biology.protein Molecular Medicine |
| Zdroj: | Molecules, Vol 17, Iss 8, Pp 8753-8761 (2012) Molecules Volume 17 Issue 8 Pages 8753-8761 |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules17088753 |
| Popis: | Vanillin was extracted from vanilla beans using pretreatment with cellulase to produce enzymatic hydrolysis, and response surface methodology (RSM) was applied to optimize the processing parameters of this extraction. The effects of heating time, enzyme quantity and temperature on enzymatic extraction of vanillin were evaluated. Extraction yield (mg/g) was used as the response value. The results revealed that the increase in heating time and the increase in enzyme quantity (within certain ranges) were associated with an enhancement of extraction yield, and that the optimal conditions for vanillin extraction were: Heating time 6 h, temperature 60 °C and enzyme quantity 33.5 mL. Calculated from the final polynomial functions, the optimal response of vanillin extraction yield was 7.62 mg/g. The predicted results for optimal reaction conditions were in good agreement with experimental values. |
| Databáze: | OpenAIRE |
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