Use of a highly sensitive quantitative telomerase assay in intracytoplasmic sperm injection programmes for the treatment of 47,XXY non-mosaic Klinefelter men
| Autor: | X. Yiannakopoulos, Apostolos Kaponis, Nikolaos Sofikitis, D. Giannakis, Ikuo Miyagawa, Y. Yamamoto, Y. Mio, A. Chatzikyziakidou, D. Loutradis, Ch. Mamoulakis, J. Georgiou |
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| Rok vydání: | 2002 |
| Předmět: |
Telomerase/*metabolism
Testis/pathology Adult Male endocrine system Telomerase Urology medicine.medical_treatment Klinefelter Syndrome/*genetics/therapy Biopsy Aneuploidy Biology Testicle Sensitivity and Specificity Intracytoplasmic sperm injection Andrology Endocrinology Klinefelter Syndrome Testis medicine Humans Sperm Injections Intracytoplasmic In Situ Hybridization Fluorescence Spermatogonium Spermatogenic Cell Spermatozoon urogenital system Mosaicism General Medicine Middle Aged medicine.disease medicine.anatomical_structure Karyotyping Female Klinefelter syndrome |
| Zdroj: | Andrologia. 34(4) |
| ISSN: | 0303-4569 |
| Popis: | We evaluated the role of the sensitive quantitative telomerase assay (SQTA) in the management of men with non-mosaic Klinefelter's syndrome (KS). Diagnostic testicular biopsy (DTB) was performed in 24 men with KS. A part of the DTB was stained and the remaining fragment was processed for the SQTA. After 3-18 months, a therapeutic testicular biopsy (TTB) was performed in the same testicle and the recovered specimens were processed to identify spermatozoa. Men with a SQTA outcome equal to 0.00 Units microg-1 protein (n = 7) demonstrated therapeutic testicular biopsy material that was negative for spermatogenic cells. In five men with a SQTA outcome of 8.11-38.03 Units microg-1, the most advanced germ cell was the spermatogonium/primary spermatocyte. In the remaining 12 men, the most advanced spermatogenic cell in the TTB was the spermatozoon. In these men, the SQTA outcome was equal to 25.76-92.68 Units microg-1 protein. Using 39.00 Units microg-1 protein as a cut-off value, the accuracy of the SQTA in identifying men positive for spermatozoa was 91.6%. It appears that the SQTA has a role for identifying non-mosaic KS men who have testicular spermatozoa. Andrologia |
| Databáze: | OpenAIRE |
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