Insulin-like effects of vanadate and selenate on the expression of glucose-6-phosphate dehydrogenase and fatty acid synthase in diabetic rats
Autor: | E A, Berg, J Y, Wu, L, Campbell, M, Kagey, S R, Stapleton |
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Rok vydání: | 1995 |
Předmět: |
Blood Glucose
Male medicine.medical_specialty medicine.medical_treatment Gene Expression Glucosephosphate Dehydrogenase Selenic Acid Weight Gain Biochemistry Selenate Diabetes Mellitus Experimental Rats Sprague-Dawley chemistry.chemical_compound Diabetes mellitus Internal medicine medicine Glucose-6-phosphate dehydrogenase Animals Hypoglycemic Agents Insulin Vanadate RNA Messenger Selenium Compounds chemistry.chemical_classification biology Fatty Acids General Medicine medicine.disease Rats Fatty acid synthase Endocrinology Enzyme Mechanism of action chemistry biology.protein medicine.symptom Vanadates |
Zdroj: | Biochimie. 77(12) |
ISSN: | 0300-9084 |
Popis: | Insulin is capable of regulating cellular and metabolic processes as well as gene expression. In recent years, enthusiasm has surfaced for using insulin-mimetics to study the mechanism of action of insulin. Vanadate and selenate are two compounds that have been found to mimic the action of insulin on control of blood glucose levels in vivo. Vanadate has also been shown to regulate the expression of several enzymes both in vivo and in vitro, however, studies concerning selenate's ability to regulate expression have not been reported. In this study we show that administration of vanadate or selenate to streptozotocin-induced diabetic rats not only normalizes blood glucose levels similarly to insulin but also positively affects the expression of two key metabolic enzymes, glucose-6-phosphate dehydrogenase (G6PDH) and fatty acid synthase (FAS). Both G6PDH and FAS activity are significantly decreased in diabetic animals compared to non-diabetic control. Treatment of the diabetic animals with either insulin, vanadate or selenate restored both activities to about 80-90% of control. All treatment conditions exhibited activities significantly higher than those determined for the diabetic group but did not differ significantly from each other. Increases in G6PDH or FAS activity are due to increases in mRNA level. Increase in both G6PDH and FAS mRNA was comparable to the observed increase in activity suggesting that regulation of expression by the mimetics occurs pretranslationally. |
Databáze: | OpenAIRE |
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