Lipopolysaccharide (LPS) Alters Phosphatidylcholine Metabolism in Elicited Peritoneal Macrophages
| Autor: | Peter A. Kiener, Nick J. Allegretto, Robert I. Grove, Glenn Warr |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Lipopolysaccharides
Time Factors Lipopolysaccharide medicine.medical_treatment Immunology Phospholipid Biology Tritium Choline Mice chemistry.chemical_compound Phosphatidylcholine medicine Animals Immunology and Allergy Macrophage Peritoneal Cavity Cells Cultured Macrophages Cell Biology Metabolism Cytokine chemistry Biochemistry Phospholipases Phosphatidylcholines Female lipids (amino acids peptides and proteins) Tumor necrosis factor alpha Phosphorus Radioisotopes |
| Zdroj: | Journal of Leukocyte Biology. 48:38-42 |
| ISSN: | 1938-3673 0741-5400 |
| DOI: | 10.1002/jlb.48.1.38 |
| Popis: | We investigated the effects of LPS on mouse peritoneal macrophage phospholipids using radiolabeled precursors. LPS (200 ng/ml) stimulated incorporation of [32P] into all classes of phospholipids within 0.5 hr, and after 2 hr the increase was 60% greater than controls. Separation of the phospholipid classes by thin-layer chromatography revealed a selective increase in incorporation of label into phosphatidylcholine (PC) (90% increase compared to approximately 50% in the other phospholipids). In macrophages labeled with [3H]-choline, LPS stimulated both the incorporation of label into PC and the release of incorporated label into the medium. The time dependencies of stimulated [3H] release and [32P] incorporation were similar. These data are consistent with the hypothesis that LPS activates macrophages via a PC-specific phospholipase-dependent mechanism. |
| Databáze: | OpenAIRE |
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