The effect of membrane fluidity on FRET parameters: an energy transfer study inside small unilamellar vesicle

Autor:	Chiranjib Ghatak, Rajib Pramanik, Nilmoni Sarkar, Souravi Sarkar, Vishal Govind Rao
Rok vydání:	2011
Předmět:	Förster resonance energy transfer Dynamic light scattering Chemistry Bilayer Vesicle Membrane fluidity Analytical chemistry General Physics and Astronomy Physical and Theoretical Chemistry Time-resolved spectroscopy Lipid bilayer Fluorescence spectroscopy
Zdroj:	Phys. Chem. Chem. Phys.. 13:3711-3720
ISSN:	1463-9084 1463-9076
DOI:	10.1039/c0cp01925a
Popis:	The fluorescence resonance energy transfer (FRET) in a lipid bilayer system containing two different donors and one common acceptor at below and above transition temperature has been studied and all the FRET parameters are analyzed using steady state and time-resolved fluorescence spectroscopy. Using dynamic light scattering measurement, we have followed the process of preparation of small unilamellar vesicles, and by following the FRET parameters of C-153-Rh6G and C-151-Rh6G pairs inside SUVs at 16 °C and 33 °C (T(m) = 23.9 °C) we have noticed that there is greater effect of temperature on the FRET parameters in case of the C-153-Rh6G pair than that of the C-151-Rh6G pair. Finally we have concluded that this difference is due to their different location inside the lipid bilayer in which fluidity of the long alkyl chain markedly affects the FRET parameters for C-153-Rh6G pair embedded inside a small unilamellar vesicle of size 20-50 nm.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdcd1267e0e12a12204d3caaa95d4dfd https://doi.org/10.1039/c0cp01925a Zobrazit plný text záznamu