Hepatic transport and binding of rose bengal in the presence of albumin and gamma globulin
| Autor: | V. S. Sharma, B. A. Luxon, E. L. Forker |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Physiology
Plasma protein binding Models Biological chemistry.chemical_compound Reaction rate constant Sinusoid Physiology (medical) Spectrophotometry Rose bengal medicine Animals Binding site Rose Bengal Hepatology medicine.diagnostic_test Gastroenterology Albumin Biological Transport Rats Inbred Strains Serum Albumin Bovine Gamma globulin Rats Kinetics Liver chemistry Biochemistry Female gamma-Globulins Protein Binding |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 248:G702-G708 |
| ISSN: | 1522-1547 0193-1857 |
| DOI: | 10.1152/ajpgi.1985.248.6.g702 |
| Popis: | Gamma globulin and albumin are compared with respect to their effects on the hepatic transport of rose bengal and with respect to the rates and affinities with which they bind this dye. The apparent intrinsic clearance of rose bengal is greater in the presence of albumin than in the presence of gamma globulin, and this difference increases with the protein concentration. Because the binding affinities of these proteins also differ, however, it cannot be concluded decisively that the mechanisms of dye removal are distinct. For both proteins the binding reaction rates as measured by stopped-flow spectrophotometry are much faster than the rate of convection along the sinusoid or the rate of removal of free dye by liver cells. The transport data and the binding rate constants are the basis for an extended theoretical model developed and analyzed in an accompanying report. |
| Databáze: | OpenAIRE |
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