Immunization against bovine herpesvirus-1 infection. Preliminary tests in calves with a DNA vaccine
| Autor: | Maura Ferrari, F Frigeri, Q Sardonini, Stefano Petrini, Cristina Marchini, M Lo Dico, A Tosini, Mauro Provinciali, G Castrucci, Augusto Amici, Daniela Salvatori |
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| Rok vydání: | 2003 |
| Předmět: |
viruses
Immunology Cattle Diseases medicine.disease_cause Antibodies Viral Microbiology Virus Herpesviridae DNA vaccination Viral Proteins Immunity Neutralization Tests Alphaherpesvirinae medicine Vaccines DNA Immunology and Allergy Animals Neutralizing antibody Herpesvirus 1 Bovine General Veterinary biology Viral Vaccines General Medicine Herpesviridae Infections biology.organism_classification Virology Bovine herpesvirus 1 Infectious Diseases biology.protein Cattle Immunization Antibody |
| Zdroj: | Comparative immunology, microbiology and infectious diseases. 27(3) |
| ISSN: | 0147-9571 |
| Popis: | A bovine herpesvirus-1 (BHV-1) vaccine expressing glycoprotein D, the form with the transmembrane anchor removed, was evaluated for inducing immunity in calves. The plasmid encoding gD of BHV-1 was injected three times to nine calves, using three animals for each of the following routes: intramuscularly (i.m.), intradermally (i.d.), or intranasally (i.n.). Three additional calves were given the plasmid vector only and served as unvaccinated controls. When calves were subjected to challenge infection with BHV-1, all vaccinated calves as well as the controls developed a typical severe form of infectious bovine rhinotracheitis. However, compared to the controls, the vaccinated calves showed earlier clearance of challenge virus. Moreover, the calves given the vaccine i.m. developed neutralizing antibody to BHV-1 between 21 and 42 days following the first injection of vaccine, whereas in calves vaccinated either i.d. or i.n., as well as the controls, antibody first appeared in their sera 14 days post-challenge infection. |
| Databáze: | OpenAIRE |
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