Differences in mRNA expression of adipocyte-derived factors in response to fasting, refeeding and leptin

Autor: Thierry Raclot, Fabrice Bertile
Přispěvatelé: Département Ecologie, Physiologie et Ethologie (DEPE-IPHC), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Institut Pluridisciplinaire Hubert Curien (IPHC), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS)-Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2004
Předmět:
Male
Leptin
MESH: Insulin-Like Growth Factor I
Adipose tissue
MESH: Rats
Sprague-Dawley
MESH: Eating
Energy homeostasis
Rats
Sprague-Dawley
Eating
chemistry.chemical_compound
0302 clinical medicine
Adipocyte
Urea
Resistin
MESH: Proteins
MESH: Animals
Insulin-Like Growth Factor I
mRNA level
10. No inequality
MESH: Nitrogen
Epididymis
0303 health sciences
education.field_of_study
[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]
Fasting
MESH: Adiponectin
Adipose Tissue
Intercellular Signaling Peptides and Proteins
Adiponectin
MESH: Membrane Proteins
Prolonged food deprivation
MESH: Adipose Tissue
hormones
hormone substitutes
and hormone antagonists
MESH: Hormones
Ectopic
medicine.medical_specialty
MESH: Rats
Nitrogen
MESH: Epididymis
MESH: Fasting
030209 endocrinology & metabolism
Biology
03 medical and health sciences
Internal medicine
medicine
Animals
Adiponutrin
RNA
Messenger
MESH: Intercellular Signaling Peptides and Proteins
education
Molecular Biology
MESH: Urea
MESH: RNA
Messenger
030304 developmental biology
Membrane Proteins
Proteins
MESH: Leptin
Cell Biology
MESH: Male
MESH: Resistin
Rats
Endocrinology
chemistry
Hormones
Ectopic
Macrophage migration inhibitory factor
Zdroj: Biochimica et Biophysica Acta-Molecular Cell Research
Biochimica et Biophysica Acta-Molecular Cell Research, Elsevier, 2004, 1683 (1-3), pp.101-109. ⟨10.1016/j.bbalip.2004.05.001⟩
ISSN: 1388-1981
0167-4889
Popis: International audience; The present study examines whether and to what extent the profiles of adipose-derived factors are altered in epididymal and subcutaneous adipose tissues of long-term fasted/refed and of fasted rats treated by recombinant leptin. Fasting was characterized by three successive metabolic phases. Minor differences in the time-course and magnitude of response were detected between the two adipose sites. Leptin, adiponectin, resistin, adiponutrin, and insulin-like growth factor-1 (IGF-1) gene expressions differentially decreased according to the fasting duration. mRNA levels reached a minimum in late fasting for these secreted factors, being decreased by 60-90% for adiponectin, resistin, and IGF-1, 95-98% for leptin and by 100% for adiponutrin. Refeeding partially or totally restored their mRNA expression in epididymal adipose. Expression levels of apolipoprotein E (ApoE), angiotensinogen (AGT), adipsin and macrophage migration inhibitory factor (MIF) were either unchanged or slightly affected. In leptin-treated rats, leptin mRNA concentrations were significantly decreased in phase 2 of fasting (by 85%) from levels in control phosphate-buffered saline (PBS)-treated rats in both tissues. Leptin treatment also decreased resistin mRNA levels (by 78% in P2L and 63% in P3L relative to control groups) in subcutaneous adipose. These data suggest that adiponectin, resistin, adiponutrin, and IGF-1 could be involved in overall energy homeostasis during prolonged fasting, as leptin is. The mechanisms that underlie the expressions of these adipose-secreted factors remain to be determined.
Databáze: OpenAIRE
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