High-Resolution Structures of DNA Minidumbbells Comprising Type II Tetraloops with a Purine Minor Groove Residue

Autor:	Cheuk Kit Ngai, Hung Kay Lee, Sik Lok Lam, Pei Guo
Rok vydání:	2020
Předmět:	Purine Base Sequence Stereochemistry DNA Genome Surfaces Coatings and Films Thymine chemistry.chemical_compound Residue (chemistry) chemistry Purines Materials Chemistry Nucleic Acid Conformation Physical and Theoretical Chemistry Trinucleotide repeat expansion Purine metabolism Groove (joinery)
Zdroj:	The Journal of Physical Chemistry B. 124:5131-5138
ISSN:	1520-5207 1520-6106
Popis:	Minidumbbell (MDB) is a newly discovered DNA structure formed by native sequences, which serves as a possible structural intermediate causing repeat expansion mutations in the genome and also a functional structural motif in constructing DNA-based molecular switches. Until now, all the reported MDBs containing two adjacent type II tetraloops were formed by pyrimidine-rich sequences 5'-YYYR YYYR-3' (Y and R represent pyrimidine and purine, respectively), wherein the second and sixth residues folded into the minor groove and interacted with each other. In this study, we have conducted a high-resolution nuclear magnetic resonance (NMR) spectroscopic investigation on alternative MDB-forming sequences and discovered that an MDB could also be formed stably with a purine in the minor groove, which has never been observed in any previously reported DNA type II tetraloops. Our refined NMR solution structures of the two MDBs formed by 5'-CTTG C
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdc38bdb2b638ece1def5c102ad5a686 https://doi.org/10.1021/acs.jpcb.0c03163 Zobrazit plný text záznamu