Involvement of UTR-dependent gene expression in the maintenance of cancer stem cell like phenotypes
Autor: | Motoaki Yasuda, Hiroki Shirato, Tomoyuki Hatanaka, Takeshi Nishioka |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
c-fos
Cancer Research cancer stem cell repopulation Ets2 Cell Population Biology untranslated region Cancer stem cell medicine Fibrosarcoma education Transcription factor education.field_of_study Oncogene Articles Cell cycle medicine.disease Cell biology medicine.anatomical_structure Oncology radio-resistance hypoxia-inducible factor-1 alpha Immunology Cancer cell |
Zdroj: | Oncology letters. 10(5):3171-3176 |
ISSN: | 1792-1074 |
Popis: | The present study demonstrated the acquisition of additional malignant characteristics in irradiated mouse fibrosarcoma cells compared with the parent cells. Several reporter assays indicated that hypoxia-inducible factor (HIF)-1 alpha, activator protein-1 and Ets-dependent transcription were activated in irradiated cells. The cis-elements in the 5'-untranslated region (UTR) of these transcription factors plays a major role in their expression in surviving irradiated cancer cells. By contrast, there were no evident differences between the 3'-UTR-dependent repression demonstrated by parent cells and irradiated cells. A small population of parental fibrosarcoma cells was also found to exhibit the same enhanced 5'-UTR-dependent HIF-1 alpha expression as that demonstrated by irradiated cells. These observations may indicate that high-dose X-ray irradiation affects the majority of proliferating cancer cells, but not the cancer stem cells (CSCs), and an increased CSC population may explain the progressive phenotypes of the irradiated cells. It appears likely that the transcription factors that maintain stemness are regulated by the same 5'-UTR-dependent mechanism. |
Databáze: | OpenAIRE |
Externí odkaz: |