A Leydig cell tumour A model for the study of lutropin action
| Autor: | M. van Driel, B.A. Cooke, L.M. Lindh, M.P.I. Van Der Plank, H.J. van der Molen, C.P. Bakker, F.H.A. Janszen |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Biophysics Endogeny Biology Leydig cell tumour Models Biological Biochemistry Testicular Neoplasms Internal medicine Cyclic AMP medicine Animals Testosterone Protein kinase A Molecular Biology Leydig cell Leydig Cells Neoplasms Experimental Metabolism Luteinizing Hormone medicine.disease Neoplasm Proteins Rats medicine.anatomical_structure Endocrinology Pregnenolone Phosphorylation Protein Kinases hormones hormone substitutes and hormone antagonists Leydig Cell Tumor medicine.drug |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 583:320-331 |
| ISSN: | 0304-4165 |
| Popis: | The properties of cells isolated from a Leydig cell tumour have been compared with normal rat testis Leydig cells. These cells were found to be similar in the following respects: 1. Lutropin-stimulated cyclic AMP and testosterone production. 2. Lutropin-activated protein kinase activity followed by phosphorylation of endogenous proteins of mol. wts. 57,000and 14,000. 3. Parallel lutropin dose vs. response curves for phosphorylation of the endogenous proteins and for testosterone production. 4. Two forms of isoenzyme, cyclic AMP dependent protein kinase, present. They differed mainly with respect to the lutropin-stimulated testosterone production, which was much lower in the tumour cells compared with the normal adult testis Leydig cells (4.6 +/- 1.1 and 114 +/- 16 ng testosterone/10(6) cells per 2 h, respectively). However, the lutropin-stimulated steroid production in the tumour cells was quantitatively comparable with the normal rat Leydig cell when the metabolism of pregnenolone in intact cells and mitochondria was inhibited by addition of SU-10603 and/or cyanoketone. It is concluded that the Leydig cell tumour used in this study can be used to investigate certain aspects of lutropin action where large quantities of cells are required. |
| Databáze: | OpenAIRE |
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