Non-Coding Changes Cause Sex-Specific Wing Size Differences between Closely Related Species of Nasonia
| Autor: | M. Victoria Cattani, Deodoro C. S. G. Oliveira, Jonathan D. Giebel, Louis van de Zande, Eveline C. Verhulst, Monica Munoz-Torres, David W. Loehlin, Leo W. Beukeboom, Rachel Edwards, John H. Werren, Michael E. Clark |
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| Přispěvatelé: | Beukeboom lab, Van de Zande lab |
| Rok vydání: | 2010 |
| Předmět: |
COURTSHIP
0106 biological sciences Cancer Research Genome evolution animal structures lcsh:QH426-470 Positional cloning VITRIPENNIS Doublesex HYMENOPTERA Locus (genetics) Quantitative trait locus Biology 010603 evolutionary biology 01 natural sciences 03 medical and health sciences PTEROMALIDAE CHARACTERS TOMATO FRUIT Genetics Allele Molecular Biology Genetics (clinical) Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences Human evolutionary genetics CENTRAL-NERVOUS-SYSTEM biology.organism_classification GENE EVOLUTION lcsh:Genetics DROSOPHILA-MELANOGASTER Nasonia |
| Zdroj: | PLoS genetics, 6(1):1000821. PUBLIC LIBRARY SCIENCE PLoS Genetics, Vol 6, Iss 1, p e1000821 (2010) |
| ISSN: | 1553-7404 1553-7390 |
| DOI: | 10.1371/journal.pgen.1000821 |
| Popis: | The genetic basis of morphological differences among species is still poorly understood. We investigated the genetic basis of sex-specific differences in wing size between two closely related species of Nasonia by positional cloning a major male-specific locus, wing-size1 (ws1). Male wing size increases by 45% through cell size and cell number changes when the ws1 allele from N. giraulti is backcrossed into a N. vitripennis genetic background. A positional cloning approach was used to fine-scale map the ws1 locus to a 13.5 kilobase region. This region falls between prospero (a transcription factor involved in neurogenesis) and the master sex-determining gene doublesex. It contains the 5'-UTR and cis-regulatory domain of doublesex, and no coding sequence. Wing size reduction correlates with an increase in doublesex expression level that is specific to developing male wings. Our results indicate that non-coding changes are responsible for recent divergence in sex-specific morphology between two closely related species. We have not yet resolved whether wing size evolution at the ws1 locus is caused by regulatory alterations of dsx or prospero, or by another mechanism. This study demonstrates the feasibility of efficient positional cloning of quantitative trait loci (QTL) involved in a broad array of phenotypic differences among Nasonia species. |
| Databáze: | OpenAIRE |
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