Alternative splicing and transcription elongation in plants
| Autor: | Alberto R. Kornblihtt, Micaela A. Godoy Herz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Mini Review
RNA polymerase II Plant Science lcsh:Plant culture Ciencias Biológicas purl.org/becyt/ford/1 [https] 03 medical and health sciences Exon alternative splicing 0302 clinical medicine Transcription (biology) Arabidopsis lcsh:SB1-1110 PLANTS ALTERNATIVE SPLICING TRANSCRIPTION purl.org/becyt/ford/1.6 [https] Gene 030304 developmental biology 0303 health sciences biology plants Alternative splicing Bioquímica y Biología Molecular biology.organism_classification Cell biology Elongation factor LIGHT RNA splicing biology.protein RNAPII transcription light 030217 neurology & neurosurgery CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Frontiers in Plant Science, Vol 10 (2019) Frontiers in Plant Science |
| DOI: | 10.3389/fpls.2019.00309/full |
| Popis: | Alternative splicing and transcription elongation by RNA polymerase II (RNAPII) are two processes which are tightly connected. Splicing is a co-transcriptional process, and different experimental approaches show that splicing is coupled to transcription in Drosophila, yeast and mammals. However, little is known about coupling of transcription and alternative splicing in plants. The kinetic coupling explains how changes in RNAPII elongation rate influence alternative splicing choices. Recent work in Arabidopsis shows that expression of a dominant negative transcription elongation factor, TFIIS, enhances exon inclusion. Furthermore, the Arabidopsis transcription elongation complex has been recently described, providing new information about elongation factors that interact with elongating RNAPII. Light regulates alternative splicing in plants through a chloroplast retrograde signaling. We have recently shown that light promotes RNAPII elongation in the affected genes, while in darkness elongation is lower. These changes in transcription are consistent with elongation causing the observed changes in alternative splicing. Altogether, these findings provide evidence that coupling between transcription and alternative splicing is an important layer of gene expression regulation in plants. Fil: Godoy Herz, Micaela Amalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Kornblihtt, Alberto Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina |
| Databáze: | OpenAIRE |
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