DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats
| Autor: | Nevena Mihailovic-Stanojevic, Jelica Grujic-Milanovic, Djurdjica Jovovic, Biljana Spremo-Potparević, Marija Bruic, Lada Zivkovic, Danijela Karanovic, Zoran Miloradovic |
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| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Antioxidant DNA damage medicine.medical_treatment Blood Pressure 030204 cardiovascular system & hematology Protein oxidation medicine.disease_cause Biochemistry Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Rats Wistar Comet assay 030304 developmental biology 0303 health sciences Kidney Cell Biology Malondialdehyde Rats 3. Good health Oxidative Stress Endocrinology medicine.anatomical_structure chemistry Oxidative stress Spontaneously hypertensive rats Hypertension |
| Zdroj: | International Journal of Biochemistry and Cell Biology The International Journal of Biochemistry & Cell Biology |
| Popis: | Oxidative damage to protein and lipid macromolecules in target organs in hypertension has been recognized as a major factor contributing to cardiovascular, cerebrovascular, and renal diseases. Data on protein and lipid oxidative damage in spontaneously hypertensive rats are numerous, but there is no information on DNA damage in tissues measured by comet assay. The aim of this study was to determine the baseline damage to DNA, protein, and lipid macromolecules in different organs of spontaneously hypertensive rats. Markers of lipid peroxidation, protein oxidation, and DNA damage were measured in blood, heart, kidney, and liver of 24-week-old spontaneously hypertensive rats. Plasma prooxidant and antioxidant status were determined as well. Age-matched normotensive Wistar rats were used as control. A rise in markers of lipid peroxidation and protein oxidation, malondialdehyde, and advanced oxidation protein products, was detected in all tissues of spontaneously hypertensive rats, with particularly high values in the liver. DNA damage, measured by the comet assay, was significantly higher in all the studied tissues of spontaneously hypertensive rats compared to normotensive control, with more severe damage in the cardiac and renal cells. Significant depletion of the plasma antioxidant barrier in spontaneously hypertensive rats was also observed. This study showed increased damage to all macromolecules in all studied samples of spontaneously hypertensive rats in comparison with control Wistar rats. |
| Databáze: | OpenAIRE |
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