Impact of in ovo administered pioneer colonizers on intestinal proteome on day of hatch
| Autor: | W.N. Briggs, Lisa Bielke, Denise Russi Rodrigues, K.M. Wilson, K.M. Chasser, A.F. Duff, W. G. Bottje |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Proteome
medicine.medical_treatment Genetics and Molecular Biology In ovo medicine.disease_cause Proinflammatory cytokine Superoxide dismutase Andrology medicine Animals lcsh:SF1-1100 Gastrointestinal tract biology Oncostatin M Interleukin General Medicine Bacterial Load Gastrointestinal Microbiome Gastrointestinal Tract Cytokine inflammation biology.protein Animal Science and Zoology lcsh:Animal culture pioneer colonizers Chickens Oxidative stress |
| Zdroj: | Poultry Science Poultry Science, Vol 99, Iss 3, Pp 1254-1266 (2020) |
| ISSN: | 1525-3171 0032-5791 |
| Popis: | Pioneer colonization of the gastrointestinal tract (GIT) by bacteria is thought to have major influence on neonatal tissue development. Previous studies have shown in ovo inoculation of embryos with saline (S), species of Citrobacter (C, C2), or lactic acid bacteria (L) resulted in an altered microbiome on day of the hatch (DOH). The present study investigated GIT proteomic changes at DOH in relation to different inoculations. Embryos were inoculated in ovo with S or ∼102 cfu of C, C2, or L at 18 embryonic days. On DOH, the GIT was collected, and tissue proteins were extracted for analysis via tandem mass spectrometry. A total of 493 proteins were identified for differential comparison with S at P ≤ 0.10. Different levels were noted in 107, 39, and 78 proteins in C, C2, and L groups, respectively, which were uploaded to Ingenuity Pathway Analysis to determine canonical pathways and biological functions related to these changes. Three members of the cytokine family (interleukin [IL]-1β, IL6, and Oncostatin M) were predicted to be activated in C2, indicated with Z-score ≥ 1.50, which suggested an overall proinflammatory GIT condition. This was consistent with the activation of the acute-phase response signaling pathway seen exclusively in C2 (Z-score = 2.00, P |
| Databáze: | OpenAIRE |
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