Enhancement of Dendrimer-Mediated Transfection Using Synthetic Lung Surfactant Exosurf Neonatal in Vitro
| Autor: | Jolanta F. Kukowska-Latallo, James R. Baker, Anna U. Bielinska, Jon Eichman, Chunling Chen |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Cell Membrane Permeability
Swine Phosphorylcholine Green Fluorescent Proteins Biophysics Gene Expression Bronchi Endosomes Biology Transfection Biochemistry Jurkat cells Polyethylene Glycols Cell membrane Jurkat Cells Surface-Active Agents Pulmonary surfactant medicine Animals Humans Luciferase Luciferases Molecular Biology Tyloxapol In Situ Hybridization Gene Transfer Techniques Pulmonary Surfactants Cell Biology Molecular biology In vitro Cell biology Drug Combinations Luminescent Proteins medicine.anatomical_structure Cell culture Endothelium Vascular Fatty Alcohols medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 264:253-261 |
| ISSN: | 0006-291X |
| Popis: | Pulmonary surfactants enhance adenovirus-mediated gene transfer but inhibit cationic liposome-mediated transfection in lung epithelial cells in vitro. This study examines the effect of the synthetic lung surfactant Exosurf on dendrimer-mediated transfection in eukaryotic cells. Exosurf significantly enhanced dendrimer-luciferase plasmid transfection in a number of cell lines and was very effective in primary cells. Luciferase expression increased up to 40-fold in primary normal human bronchial/tracheal epithelial cells (NHBE). FACScan analysis demonstrated that the transfection rate of the human T cell leukemia Jurkat cell line has significantly improved from 10 to 90% of cells at 24 h after transfection. Analysis of the components of Exosurf revealed that the nonionic surfactant tyloxapol was responsible for the enhancement of dendrimer-mediated gene transfer. The tyloxapol effect was due to increased cell membrane porosity and DNA uptake. Our results demonstrate that Exosurf and its component, tyloxapol, constitute a powerful enhancer for dendrimer-mediated gene transfer in vitro. |
| Databáze: | OpenAIRE |
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