Chitosan-mediated in situ biomolecule assembly in completely packaged microfluidic devices
| Autor: | Gary W. Rubloff, Xiaolong Luo, Reza Ghodssi, Hyunmin Yi, William E. Bentley, Gregory F. Payne, Jung Jin Park, Theresa Michelle Valentine |
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| Rok vydání: | 2006 |
| Předmět: |
chemistry.chemical_classification
In situ Chitosan Materials science Fabrication Biomolecule Microfluidics Biomedical Engineering Bioengineering Nanotechnology Biosensing Techniques General Chemistry Microfluidic Analytical Techniques Biochemistry chemistry.chemical_compound chemistry Electrode Electrochemistry Fluidics Gold Electrodes Biosensor |
| Zdroj: | Lab on a Chip. 6:1315 |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/b603101c |
| Popis: | We report facile in situ biomolecule assembly at readily addressable sites in microfluidic channels after complete fabrication and packaging of the microfluidic device. Aminopolysaccharide chitosan's pH responsive and chemically reactive properties allow electric signal-guided biomolecule assembly onto conductive inorganic surfaces from the aqueous environment, preserving the activity of the biomolecules. A transparent and nonpermanently packaged device allows consistently leak-free sealing, simple in situ and ex situ examination of the assembly procedures, fluidic input/outputs for transport of aqueous solutions, and electrical ports to guide the assembly onto the patterned gold electrode sites within the channel. Both in situ fluorescence and ex situ profilometer results confirm chitosan-mediated in situ biomolecule assembly, demonstrating a simple approach to direct the assembly of biological components into a completely fabricated device. We believe that this strategy holds significant potential as a simple and generic biomolecule assembly approach for future applications in complex biomolecular or biosensing analyses as well as in sophisticated microfluidic networks as anticipated for future lab-on-a-chip devices. |
| Databáze: | OpenAIRE |
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