Extract from the Coriolus versicolor Fungus as an Anti-Inflammatory Agent with Cytotoxic Properties against Endothelial Cells and Breast Cancer Cells
Autor: | Sylwia Wrotek, Małgorzata Pawlikowska, Justyna Sobocińska, Artur Dzialuk, Tomasz Jędrzejewski |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Lipopolysaccharide
Inflammation Catalysis lcsh:Chemistry Inorganic Chemistry chemistry.chemical_compound medicine Cytotoxic T cell Viability assay Coriolus versicolor extract Physical and Theoretical Chemistry Cytotoxicity lcsh:QH301-705.5 Molecular Biology Spectroscopy Organic Chemistry lipopolysaccharide Interleukin General Medicine Toll-like receptor 4 endothelial cells Computer Science Applications lcsh:Biology (General) lcsh:QD1-999 chemistry phosphorylated IκB inflammation Cancer cell TLR4 Cancer research medicine.symptom breast cancer cells |
Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 23 International Journal of Molecular Sciences, Vol 21, Iss 9063, p 9063 (2020) |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms21239063 |
Popis: | Chronic inflammation is a well-recognised tumour-enabling component, which includes bioactive molecules from cells infiltrating the tumour microenvironment and increases the risk of cancer progression. Since long-term use of the currently available anti-inflammatory drugs used in cancer therapy causes numerous side effects, the aim of this study was to investigate the effect of an extract isolated from the Coriolus versicolor fungus (CV extract) on HUVEC endothelial cells and MCF-7 breast cancer cells in a pro-inflammatory microenvironment mimicked by lipopolysaccharide (LPS). The cells were simultaneously stimulated with the LPS and CV extract. After co-treatment, the cell viability, generation of reactive oxygen species (ROS), wound-healing assay, production of the pro-inflammatory and pro-angiogenic factors (interleukin (IL) 6, IL-8, and metalloproteinase (MMP) 9)), as well as expression of Toll-like receptor (TLR) 4 and phosphorylated I&kappa B (p-I&kappa B) were evaluated. The results showed that the CV extract inhibited IL-6, IL-8, and MMP-9 production by the LPS-stimulated cells. This effect was accompanied by a decrease in TLR4 and p-I&kappa B expression. The CV extract also had anti-migratory properties and induced a cytotoxic effect on the cells that was enhanced in the presence of LPS. The observed cytotoxicity was associated with an increase in ROS generation. We conclude that the CV extract possesses cytotoxic activity against cancer cells and endothelial cells and has the ability to inhibit the expression of the pro-tumorigenic factors associated with inflammation. |
Databáze: | OpenAIRE |
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