Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture
| Autor: | Adrián Jordá, Martin Aldasoro, Ignacio Campo Palacio, Jose Mª Vila, Contanza Aldasoro, Juan Campos Campos, Carlos Colmena, Sandeep Kumar Singhb, Elena Obrador, Soraya L. Valles |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Blood Glucose
ranolazine insulin astrocytes inflammation antioxidants Superoxide Dismutase Sistema nerviós central Malalties Organic Chemistry Anti-Inflammatory Agents NF-kappa B endocrinology_metabolomics General Medicine Catalysis Antioxidants Computer Science Applications PPAR gamma Inorganic Chemistry Cyclooxygenase 2 Ranolazine Astrocytes Insulin Regular Human Insulin Physical and Theoretical Chemistry Proto-Oncogene Proteins c-akt Molecular Biology Spectroscopy |
| Zdroj: | Jordá Vallés, Adrián Aldasoro Celaya, Martín Campo Palacio, Ignacio Vila Salinas, José María Aldasoro, Constanza Campos Campos, Juan Colmena Zaragoza, Carlos Manuel Singh, Sandeep Kumar Obrador, Elena Valles Martí, Lilian Soraya 2022 Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture International Journal Of Molecular Sciences 23 RODERIC. Repositorio Institucional de la Universitat de Valéncia instname International Journal of Molecular Sciences; Volume 23; Issue 19; Pages: 11969 |
| Popis: | Ranolazine (Rn) is a drug used to treat persistent chronic coronary ischemia. It has also been shown to have therapeutic benefits on the central nervous system and an anti-diabetic effect by lowering blood glucose levels and however, no effects of Rn on cellular sensitivity to insulin (Ins) have been demonstrated yet. The present study aimed to investigate the permissive effects of Rn on the actions of Ins in astrocytes in primary culture. Ins at 10-8 M, Rn (10-6 M) and Ins+Rn (10-8 M and 10−6 M respectively) were added to astrocytes during 24 h. In comparison to control cells, Rn and/or Ins caused modifications in cell viability and proliferation. p-AKT, p-ERK, p-eNOS, Mn-SOD, COX-2, and the anti-inflammatory protein COX-2 were all upregulated by ins. On the contrary, no significant changes were found in the protein expression of Cu/Zn-SOD, NF-κB and IκB. The presence of Rn produced an increase in p-ERK protein and a significant decrease in COX-2 protein expression. Furthermore, Rn significantly increased the effects of Ins on the expression of p-AKT, p-eNOS, p-ERK, Mn-SOD, and PPAR-γ. On the other hand, Rn+Ins produced a significant decrease in COX-2 expression. In conclusion, Rn facilitated the effects of insulin on the p-AKT, p-eNOS, p-ERK, Mn-SOD and PPAR-γ, signaling pathways, as well as on the anti-inflammatory and antioxidant effects of the hormone. |
| Databáze: | OpenAIRE |
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