Intracellular Distribution of Pancreatic Tumor Antigen Purified from BHP-Induced Adenocarcinomas in Syrian Hamsters
| Autor: | Shunji Kitazawa, Tsuneo Natori, Masaharu Matsuzaki, Kazuhiro Mizumoto, Masahiro Tsutsumi, Yoichi Konishi |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Nitrosamines Endocrinology Diabetes and Metabolism Immunocytochemistry Hamster Adenocarcinoma urologic and male genital diseases Endocrinology Antigen Antigens Neoplasm Pancreatic tumor Cricetinae Internal medicine Biomarkers Tumor otorhinolaryngologic diseases Internal Medicine medicine Animals Amino Acids Mesocricetus Hepatology biology Chemistry medicine.disease Molecular biology Epithelium Pancreatic Neoplasms body regions surgical procedures operative medicine.anatomical_structure biology.protein Antibody Pancreas |
| Zdroj: | Pancreas. 4:429-435 |
| ISSN: | 0885-3177 |
| DOI: | 10.1097/00006676-198908000-00006 |
| Popis: | Pancreatic tumor antigen (PTA) was purified to apparent homogeneity from hamster pancreatic adenocarcinomas by a rapid and simple procedure using column chromatography on Mono P and Superose 6. Amino acid analysis indicated essential similarities between hamster PTA and human pancreatic cancer-associated antigen (PCAA, PCAAc), although differences in the contents of several amino acid residues between the two proteins did suggest species variation. Investigation of the binding pattern of antibody raised against PTA by peroxidase-labeled antibody immunocytochemistry revealed that, whereas PTA is absent or only faintly expressed in normal epithelium, it is very strongly positive in transplantable hamster pancreatic adenocarcinomas induced by nitrosamines. PTA was found to be distributed over the entire surface of neoplastic cells and it was concluded that the presence of PTA on the basolateral surface of malignant pancreatic epithelial cells, where it might have access to the blood circulation, could be one explanation for the observed elevated concentrations of PTA in the serum. PTA is thus a potential marker for hamster neoplastic pancreatobiliary duct-type cells. |
| Databáze: | OpenAIRE |
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