Triple Contrast CT Method Enables Simultaneous Evaluation of Articular Cartilage Composition and Segmentation
| Autor: | Annina E. A. Saukko, Goran Lovric, Wujun Xu, Mark W. Grinstaff, Vesa-Pekka Lehto, Mikael J. Turunen, Juuso T. J. Honkanen, Miitu K. M. Honkanen, Juha Töyräs |
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| Předmět: |
Cartilage
Articular media_common.quotation_subject mechanical-properties 0206 medical engineering Biomedical Engineering computed-tomography Contrast Media Computed tomography Articular cartilage 02 engineering and technology enhanced ct in-vivo CIBM-PC 030218 nuclear medicine & medical imaging contrast-enhanced computed tomography 03 medical and health sciences 0302 clinical medicine medicine Contrast (vision) Animals Segmentation post-traumatic osteoarthritis dual contrast agent media_common degradation synchrotron microct biology Gadoteridol medicine.diagnostic_test equilibrium distribution Chemistry Cartilage diffusion triple contrast agent computed tomography agent Patella X-Ray Microtomography 020601 biomedical engineering trypsin treatment Bovine Cartilage medicine.anatomical_structure Proteoglycan x-ray biology.protein Original Article Cattle medicine.drug Biomedical engineering |
| Zdroj: | Annals of Biomedical Engineering |
| Popis: | Early degenerative changes of articular cartilage are detected using contrast-enhanced computed tomography (CT) with a cationic contrast agent (CA). However, cationic CA diffusion into degenerated cartilage decreases with proteoglycan depletion and increases with elevated water content, thus hampering tissue evaluation at early diffusion time points. Furthermore, the contrast at synovial fluid-cartilage interface diminishes as a function of diffusion time hindering accurate cartilage segmentation. For the first time, we employ quantitative dual-energy CT (QDECT) imaging utilizing a mixture of three CAs (cationic CA4+ and non-ionic gadoteridol which are sensitive to proteoglycan and water contents, respectively, and bismuth nanoparticles which highlight the cartilage surface) to simultaneously segment the articulating surfaces and determine of the cartilage condition. Intact healthy, proteoglycan-depleted, and mechanically injured bovine cartilage samples (n = 27) were halved and imaged with synchrotron microCT 2-h post immersion in triple CA or in dual CA (CA4+ and gadoteridol). CA4+ and gadoteridol partitions were determined using QDECT, and pairwise evaluation of these partitions was conducted for samples immersed in dual and triple CAs. In conclusion, the triple CA method is sensitive to proteoglycan depletion while maintaining sufficient contrast at the articular surface to enable detection of cartilage lesions caused by mechanical impact. |
| Databáze: | OpenAIRE |
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