Behavioural food anticipation in clock genes deficient mice: confirming old phenotypes, describing new phenotypes
Autor: | Urs Albrecht, Etienne Challet, Jorge E. Mendoza |
---|---|
Přispěvatelé: | Institut des Neurosciences Cellulaires et Intégratives (INCI), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
Rok vydání: | 2010 |
Předmět: |
medicine.medical_specialty
animal structures [SDV]Life Sciences [q-bio] Photoperiod Mutant Biology Statistics Nonparametric 03 medical and health sciences Behavioral Neuroscience Mice Mice Neurologic Mutants 0302 clinical medicine Internal medicine Genetics medicine Zeitgeber Animals Circadian rhythm Gene ComputingMilieux_MISCELLANEOUS 030304 developmental biology Mice Knockout 0303 health sciences Analysis of Variance Appetitive Behavior Behavior Animal Association Learning Feeding Behavior Period Circadian Proteins Phenotype Circadian Rhythm CLOCK Cryptochromes Endocrinology Neurology Hypothalamus Anticipation (genetics) Female Suprachiasmatic Nucleus sense organs 030217 neurology & neurosurgery |
Zdroj: | Genes, Brain and Behavior Genes, Brain and Behavior, Wiley, 2010, ⟨10.1111/j.1601-183X.2010.00576.x⟩ |
ISSN: | 1601-183X 1601-1848 |
Popis: | Animals fed daily at the same time exhibit circadian food-anticipatory activity (FAA), which has been suggested to be driven by one or several food-entrainable oscillators (FEOs). FAA is altered in mice lacking some circadian genes essential for timekeeping in the main suprachiasmatic clock (SCN). Here, we confirmed that single mutations of clock genes Per1(-/-) and Per2(Brdm1) alter FAA expression in constant darkness (DD) or under a light-dark cycle (LD). Furthermore, we found that Per1(-/-);Per2(Brdm1) and Per2(Brdm1);Cry1(-/-) double mutant animals did not display a stable and significant FAA either in DD or LD. Interestingly, rescued behavioural rhythms in Per2(Brdm1);Cry2(-/-) mice in DD were totally entrained to feeding time and re-synchronized after phase-shifts of mealtime, indicating a higher SCN sensitivity to feeding cues. However, under an LD cycle and restricted feeding at midday, FAA in double Per2(Brdm1);Cry2(-/-) mutant mice was absent. These results indicate that shutting down one or two clock genes results in altered circadian meal anticipation. Moreover, we show that in a genetically rescued SCN clock (Per2(Brdm1);Cry2(-/-)), food is a powerful zeitgeber to entrain behavioural rhythms, leading the SCN to be more sensitive to feeding cues than in wild-type littermates. |
Databáze: | OpenAIRE |
Externí odkaz: |