Worms taste bitter: ASH neurons, QUI-1, GPA-3 and ODR-3 mediate quinine avoidance in Caenorhabditis elegans
| Autor: | Massimo A. Hilliard, Paolo Bazzicalupo, Ronald H.A. Plasterk, Carmela Bergamasco, Salvatore Arbucci |
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| Rok vydání: | 2004 |
| Předmět: |
Taste
G protein Protein subunit Molecular Sequence Data Sensory system GTP-Binding Protein alpha Subunits Gi-Go Biology Article General Biochemistry Genetics and Molecular Biology medicine Animals Amino Acid Sequence Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Neurons Quinine General Immunology and Microbiology General Neuroscience Neuropeptides biology.organism_classification GTP-Binding Protein alpha Subunits Cell biology Protein Subunits Phenotype medicine.anatomical_structure Solubility Biochemistry Mutation Neuron Aversive Stimulus medicine.drug |
| Zdroj: | The EMBO Journal. 23:1101-1111 |
| ISSN: | 1460-2075 0261-4189 |
| Popis: | An animal's ability to detect and avoid toxic compounds in the environment is crucial for survival. We show that the nematode Caenorhabditis elegans avoids many water-soluble substances that are toxic and that taste bitter to humans. We have used laser ablation and a genetic cell rescue strategy to identify sensory neurons involved in the avoidance of the bitter substance quinine, and found that ASH, a polymodal nociceptive neuron that senses many aversive stimuli, is the principal player in this response. Two G protein alpha subunits GPA-3 and ODR-3, expressed in ASH and in different, nonoverlapping sets of sensory neurons, are necessary for the response to quinine, although the effect of odr-3 can only be appreciated in the absence of gpa-3. We identified and cloned a new gene, qui-1, necessary for quinine and SDS avoidance. qui-1 codes for a novel protein with WD-40 domains and which is expressed in the avoidance sensory neurons ASH and ADL. |
| Databáze: | OpenAIRE |
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