Engraftment capacity of mesenchymal cells following hematopoietic stem cell transplantation in patients receiving reduced-intensity conditioning regimen
| Autor: | Attilio Olivieri, Loredana Buscemi, R Pasquini, M C Masia, E Costantino, Nadia Viola, F Balducci, Giancarlo Discepoli, Adriano Tagliabracci, Antonella Poloni, Paolo Corradini, P. Leoni |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Cancer Research Pathology medicine.medical_specialty Stromal cell Myeloid Transplantation Conditioning medicine.medical_treatment Hematopoietic stem cell transplantation Biology In Vitro Techniques Chimerism medicine Humans Transplantation Homologous Cells Cultured Aged Mesenchymal stem cell Graft Survival Hematopoietic Stem Cell Transplantation Mesenchymal Stem Cells Hematology Middle Aged Transplantation Haematopoiesis medicine.anatomical_structure Oncology Immunology Disease Progression Female Bone marrow Stem cell |
| Zdroj: | Leukemia. 20(2) |
| ISSN: | 0887-6924 |
| Popis: | The engraftment ability of mesenchymal cells was investigated in 26 patients receiving allogeneic transplantation from HLA-identical siblings with reduced-intensity conditioning (RIC). The stem cell source was bone marrow (BM) in eight patients and G-CSF-mobilized peripheral blood hematopoietic cells in 18 cases. A total of 32 patients engrafted very quickly and the chimerism evaluation (both on myeloid and on lymphoid subsets) showed that they were full donor by day 60. At the time of the study they were in complete hematological remission and displayed a full donor hematopoiesis. Two patients showed early disease progression while one did not engraft. Forty-eight out-marrow samples harvested from the 26 patients generated a marrow stromal layer adequate for the chimerism evaluation. Monocyte-macrophage contamination of marrow stromal layers was always reduced below 2% by repeated trypsinizations and treatment with the leucyl-leucine (leu-leu) methyl ester. The chimerism evaluation was performed by PCR analysis of STRs microsatellites and the amelogenin locus, by using capillary electrophoresis (CE) and by FISH analysis in case of the sex mismatch. In eight patients, a partial donor origin of stromal cells was shown (7-86% cells of donor). The source of hematopoietic cells was BM in three patients and mobilized peripheral blood in the other five. |
| Databáze: | OpenAIRE |
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