Free Extracellular Diffusion Creates the Dpp Morphogen Gradient of the Drosophila Wing Disc
| Autor: | Michelle A. Digman, Arthur D. Lander, Shaohua Zhou, Jeffrey L. Suhalim, Enrico Gratton, Qing Nie, Wing-Cheong Lo |
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| Rok vydání: | 2012 |
| Předmět: |
Embryo
Nonmammalian animal structures Diffusion Biology General Biochemistry Genetics and Molecular Biology Article Animals Genetically Modified Animals Drosophila Proteins Wings Animal Wing Decapentaplegic Agricultural and Biological Sciences(all) Ecology Biochemistry Genetics and Molecular Biology(all) Fluorescence recovery after photobleaching Transport protein Coupling (electronics) Protein Transport Spectrometry Fluorescence Larva Biophysics Drosophila General Agricultural and Biological Sciences Extracellular Space Drosophila Protein Morphogen Fluorescence Recovery After Photobleaching |
| Zdroj: | Zhou, S; Lo, WC; Suhalim, JL; Digman, MA; Gratton, E; Nie, Q; et al.(2012). Free extracellular diffusion creates the Dpp morphogen gradient of the Drosophila wing disc. Current Biology, 22(8), 668-675. doi: 10.1016/j.cub.2012.02.065. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/8cq3918b |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/j.cub.2012.02.065 |
| Popis: | Background: How morphogen gradients form has long been a subject of controversy. The strongest support for the view that morphogens do not simply spread by free diffusion has come from a variety of studies of the Decapentaplegic (Dpp) gradient of the Drosophila larval wing disc. Results: In the present study, we initially show how the failure, in such studies, to consider the coupling of transport to receptor-mediated uptake and degradation has led to estimates of transport rates that are orders of magnitude too low, lending unwarranted support to a variety of hypothetical mechanisms, such as "planar transcytosis" and "restricted extracellular diffusion." Using several independent dynamic methods, we obtain data that are inconsistent with such models and show directly that Dpp transport occurs by simple, rapid diffusion in the extracellular space. We discuss the implications of these findings for other morphogen systems in which complex transport mechanisms have been proposed. Conclusions: We believe that these findings resolve a major, longstanding question about morphogen gradient formation and provide a solid framework for interpreting experimental observations of morphogen gradient dynamics. © 2012 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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