Control of Paternally Expressed Imprinted UPWARD CURLY LEAF1, a GeneEncoding an F-Box Protein That Regulates CURLY LEAF Polycomb Protein, inthe Arabidopsis Endosperm
| Autor: | Tzung-Fu Hsieh, Yang Do Choi, Jong Seob Lee, Cheol Woong Jeong, Yeonhee Choi, Guen Tae Park, Hyein Yun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Ubiquitin-Protein Ligases
Arabidopsis Polycomb-Group Proteins lcsh:Medicine Biology DNA methyltransferase Endosperm Genomic Imprinting Gene Expression Regulation Plant Polycomb-group proteins Transgenes Imprinting (psychology) lcsh:Science Gene Alleles Genetics Multidisciplinary Base Sequence Arabidopsis Proteins F-Box Proteins lcsh:R food and beverages DNA Methylation biology.organism_classification DNA methylation lcsh:Q Genomic imprinting Research Article |
| Zdroj: | PLOS ONE(10): 2 PLoS ONE, Vol 10, Iss 2, p e0117431 (2015) PLoS ONE |
| Popis: | Genomic imprinting, an epigenetic process in mammals and flowering plants, refers to the differential expression of alleles of the same genes in a parent-of-origin-specific manner. In Arabidopsis, imprinting occurs primarily in the endosperm, which nourishes the developing embryo. Recent high-throughput sequencing analyses revealed that more than 200 loci are imprinted in Arabidopsis; however, only a few of these imprinted genes and their imprinting mechanisms have been examined in detail. Whereas most imprinted loci characterized to date are maternally expressed imprinted genes (MEGs), PHERES1 (PHE1) and ADMETOS (ADM) are paternally expressed imprinted genes (PEGs). Here, we report that UPWARD CURLY LEAF1 (UCL1), a gene encoding an E3 ligase that degrades the CURLY LEAF (CLF) polycomb protein, is a PEG. After fertilization, paternally inherited UCL1 is expressed in the endosperm, but not in the embryo. The expression pattern of a beta-glucuronidase (GUS) reporter gene driven by the UCL1 promoter suggests that the imprinting control region (ICR) of UCL1 is adjacent to a transposable element in the UCL1 5'-up-stream region. Polycomb Repressive Complex 2 (PRC2) silences the maternal UCL1 allele in the central cell prior to fertilization and in the endosperm after fertilization. The UCL1 imprinting pattern was not affected in paternal PRC2 mutants. We found unexpectedly that the maternal UCL1 allele is reactivated in the endosperm of Arabidopsis lines with mutations in cytosine DNA METHYLTRANSFERASE 1 (MET1) or the DNA glycosylase DEME-TER (DME), which antagonistically regulate CpG methylation of DNA. By contrast, maternal UCL1 silencing was not altered in mutants with defects in non-CpG methylation. Thus, silencing of the maternal UCL1 allele is regulated by both MET1 and DME as well as by PRC2, suggesting that divergent mechanisms for the regulation of PEGs evolved in Arabidopsis. |
| Databáze: | OpenAIRE |
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