Levels and Clinical Significance of Regulatory B Cells and T Cells in Acute Myeloid Leukemia

Autor: Shuli Wang, Chunyan Chen, Qiaofeng Dong, Yuqi Sang, Claudio Fozza, Xinguang Liu, Shuhuan Yang, Guosheng Li
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Adult
Male
Myeloid
Adolescent
Article Subject
Regulatory B cells
medicine.disease_cause
T-Lymphocytes
Regulatory
General Biochemistry
Genetics and Molecular Biology
Autoimmunity
03 medical and health sciences
Young Adult
0302 clinical medicine
Immune system
Transforming Growth Factor beta
hemic and lymphatic diseases
Medicine
Humans
Lymphocyte Count
RNA
Messenger
neoplasms
Aged
Aged
80 and over
B-Lymphocytes
Regulatory
General Immunology and Microbiology
business.industry
Myeloid leukemia
FOXP3
Forkhead Transcription Factors
General Medicine
Middle Aged
medicine.disease
Interleukin-10
Up-Regulation
Transplantation
Gene Expression Regulation
Neoplastic
Leukemia
Leukemia
Myeloid
Acute
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Case-Control Studies
Immunology
Female
business
Research Article
Zdroj: BioMed Research International, Vol 2020 (2020)
BioMed Research International
ISSN: 2314-6133
DOI: 10.1155/2020/7023168
Popis: Acute myeloid leukemia (AML) is a heterogeneous hematological malignancy, whose immunological mechanisms are still partially uncovered. Regulatory B cells (Bregs) and CD4+ regulatory T cells (Tregs) are subgroups of immunoregulatory cells involved in modulating autoimmunity, inflammation, and transplantation reactions. Herein, by studying the number and function of Breg and Treg cell subsets in patients with AML, we explored their potential role in the pathogenesis of AML. Newly diagnosed AML patients, AML patients in complete remission, and healthy controls were enrolled. Flow cytometry was used to detect percentages of Bregs and Tregs. ELISA was conducted to detect IL-10 and TGF-β in plasma. The mRNA levels of IL-10 and Foxp3 were measured with RT-qPCR. The relationship of Bregs and Tregs with the clinicopathological parameters was analyzed. There was a significant reduction in the frequencies of Bregs and an increase of Tregs in newly diagnosed AML patients compared with healthy controls. Meanwhile, patients in complete remission exhibited levels of Bregs and Tregs comparable to healthy controls. Furthermore, compared with healthy controls and AML patients in complete remission, newly diagnosed AML patients had increased plasma IL-10 but reduced TGF-β. IL-10 and Foxp3 mRNA levels were upregulated in the newly diagnosed AML patients. However, there were no significant differences in IL-10 and Foxp3 mRNA levels between patients in complete remission and healthy controls. Bregs and Tregs have abnormal distribution in AML patients, suggesting that they might play an important role in regulating immune responses in AML.
Databáze: OpenAIRE
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