Unraveling metabolic flexibility of rhodococci in PCB transformation

Autor: Martin-Laurent Fabrice, Udiković Kolić Nikolina, Fingler Sanja, Drevenkar Vlasta, Begonja Kolar Ana, Hršak Dubravka, Petrić Ines
Přispěvatelé: Ruđer Boˇskovi´c Institute, Division for Marine and Environmental Research, Zagreb, Croatia, Institute for Medical Research and Occupational Health, Zagreb, Croatia, Pliva, Zagreb, Croatia, Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Environmental Engineering
Stereochemistry
Health
Toxicology and Mutagenesis
[SDV]Life Sciences [q-bio]
0208 environmental biotechnology
Substituent
02 engineering and technology
010501 environmental sciences
Microbiology
01 natural sciences
Dioxygenases
chemistry.chemical_compound
bph pathway
Biotransformation
Polychlorinated biphenyls
Pleiotropy
Dioxygenase
Environmental Chemistry
Rhodococcus
Biology
Oxidative decarboxylation
0105 earth and related environmental sciences
Biphenyl
biology
Chemistry
transformation products
Public Health
Environmental and Occupational Health
multiple pathways
General Medicine
General Chemistry
biology.organism_classification
Pollution
020801 environmental engineering
Transformation (genetics)
Biodegradation
Environmental
Transformation products
Multiple pathways
biotransformation
Zdroj: Chemosphere
Chemosphere, Elsevier, 2021, 282, pp.130975. ⟨10.1016/j.chemosphere.2021.130975⟩
ISSN: 0045-6535
DOI: 10.1016/j.chemosphere.2021.130975⟩
Popis: International audience; Even though the genetic attributes suggest presence of multiple degradation pathways, most of rhodococci are known to transform PCBs only via regular biphenyl (bph) pathway. Using GC-MS analysis, we monitored products formed during transformation of 2,4,4′-trichlorobiphenyl (PCB-28), 2,2′,5,5′-tetrachlorobiphenyl (PCB-52) and 2,4,3′-trichlorobiphenyl (PCB-25) by previously characterized PCB-degrading rhodococci Z6, T6, R2, and Z57, with the aim to explore their metabolic pleiotropy in PCB transformations. A striking number of different transformation products (TPs) carrying a phenyl ring as a substituent, both those generated as a part of the bph pathway and an array of unexpected TPs, implied a curious transformation ability. We hypothesized that studied rhodococcal isolates, besides the regular one, use at least two alternative pathways for PCB transformation, including the pathway leading to acetophenone formation (via 3,4 (4,5) dioxygenase attack on the molecule), and a third sideway pathway that includes stepwise oxidative decarboxylation of the aliphatic side chain of the 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate. Structure of the identified chlorinated benzoic acids and acetophenones allowed us to hypothesize that the first two pathways were the outcome of a ring-hydroxylating dioxygenase with the ability to attack both the 2,3 (5,6) and the 3,4 (4,5) positions of the biphenyl ring as well as dechlorination activity at both, -ortho and -para positions. We propose that several TPs produced by the bph pathway could have caused the triggering of the third sideway pathway. In conclusion, this study proposed ability of rhodococci to use different strategies in PCB transformation, which allows them to circumvent potential negative aspect of TPs on the overall transformation pathway.
Databáze: OpenAIRE
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