Detection of Clostridium botulinum type G toxin by enzyme-linked immunosorbent assay
| Autor: | S S Kulinski, D W Reichard, J F Metzger, G E Lewis |
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| Rok vydání: | 1981 |
| Předmět: |
Botulinum Toxins
Clostridium botulinum type G Bacterial Toxins Enzyme-Linked Immunosorbent Assay Biology Cross Reactions medicine.disease_cause Applied Microbiology and Biotechnology Median lethal dose Microbiology Clostridium medicine Botulinum toxin type G chemistry.chemical_classification Microbial toxins Ecology Toxin biology.organism_classification Molecular biology Enzyme chemistry Alkaline phosphatase Food Science Biotechnology Research Article |
| Zdroj: | Applied and environmental microbiology. 42(6) |
| ISSN: | 0099-2240 |
| Popis: | Clostridium botulinum type G toxin was detected and quantified readily with the enzyme-linked immunosorbent assay. With the double-sandwich technique and alkaline phosphatase as the enzyme indicator, C. botulinum toxin type G was detected in quantities equaling those required for one mouse intraperitoneal median lethal dose. The time required for the procedure was approximately 6.5 h, but this requirement could have been reduced to 5.5 h or less with the use of precoated plates stored at -70 degrees C. Cross-reactions did not occur with culture extracts of C. sporogenes of C. botulinum types B, C, D, E, and F. Acidic preparations of C. botulinum type A exhibited nonspecific reactivity. Likewise, 50% of the C. subterminale isolates tested were cross-reactive in the assay. These latter isolates express similar metabolic and physiological characteristics with C. botulinum type G. |
| Databáze: | OpenAIRE |
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