(1)H NMR Metabolomics Study of Spleen from C57BL/6 Mice Exposed to Gamma Radiation
| Autor: | Jianzhi Z. Hu, Xiongjie Xiao, M. Liu, M. Hu |
|---|---|
| Předmět: |
0301 basic medicine
PCA Taurine OPLS Analytical chemistry Phenylalanine Glutathione Spectral deconvolution Biology Creatine Article 3. Good health 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Metabolomics Biochemistry chemistry Gamma radiation Valine Irradiation 1H NMR metabolomics Spleen |
| Zdroj: | ResearcherID Metabolomics : open access |
| Popis: | Due to the potential risk of accidental exposure to gamma radiation, it’s critical to identify the biomarkers of radiation exposed creatures. In the present study, NMR based metabolomics combined with multivariate data analysis to evaluate the metabolites changed in the C57BL/6 mouse spleen after 4 days whole body exposure to 3.0 Gy and 7.8 Gy gamma radiations. Principal component analysis (PCA) and orthogonal projection to latent structures analysis (OPLS) are employed for classification and identification potential biomarkers associated with gamma irradiation. Two different strategies for NMR spectral data reduction (i.e., spectral binning and spectral deconvolution) are combined with normalize to constant sum and unit weight before multivariate data analysis, respectively. The combination of spectral deconvolution and normalization to unit weight is the best way for identifying discriminatory metabolites between the irradiation and control groups. Normalized to the constant sum may achieve some pseudo biomarkers. PCA and OPLS results shown that the exposed groups can be well separated from the control group. Leucine, 2-aminobutyrate, valine, lactate, arginine, glutathione, 2-oxoglutarate, creatine, tyrosine, phenylalanine, π-methylhistidine, taurine, myoinositol, glycerol and uracil are significantly elevated while ADP is decreased significantly. These significantly changed metabolites are associated with multiple metabolic pathways and may be potential biomarkers in the spleen exposed to gamma irradiation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|