Phenotypic and functional characterization of vaginal dendritic cells in a rat model of Candida albicans vaginitis
| Autor: | M. Boccanera, Stefania Morrone, Consuelo Amantini, Silvia Arancia, Flavia De Bernardis, Antonio Cassone, Giorgio Santoni, Michela Mosca, Roberta Lucciarini |
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| Rok vydání: | 2006 |
| Předmět: |
Adoptive cell transfer
Immunology Microbiology Immunophenotyping Antigen Immunity Candida albicans Animals Rats Wistar Vaginitis Immunity Mucosal Cells Cultured Cell Proliferation biology Candidiasis Dendritic cell Dendritic Cells biology.organism_classification Adoptive Transfer Rats Infectious Diseases Vagina Parasitology Cytokine secretion Tumor necrosis factor alpha Female Lymph Nodes Fungal and Parasitic Infections CD80 |
| Zdroj: | Infection and immunity. 74(7) |
| ISSN: | 0019-9567 |
| Popis: | This study analyzes the phenotype of vaginal dendritic cells (VDCs), their antigenic presentation and activation of T-cell cytokine secretion, and their protective role in a rat model ofCandidavaginitis. Histological observation demonstrated a significant accumulation of OX62+VDCs in the mucosal epithelium ofCandida albicans-infected rats at the third round of infection. We identified two subsets of OX62+VDCs differing in the expression of CD4 molecule in both noninfected andCandida-infected rats. The OX62+CD4+subset of VDCs displayed a lymphoid cell-like morphology and expressed the T-cell antigen CD5, whereas the OX62+CD4−VDC subset exhibited a myeloid morphology and was CD5 negative.Candidainfection resulted in VDC maturation with enhanced expression of CD80 and CD134L on both CD4+and CD4−VDC subsets at 2 and 6 weeks afterCandidainfection. CD5−CD4−CD86−CD80−CD134L+VDCs from infected, but not noninfected, rats spontaneously released large amounts of interleukin-12 (IL-12) and tumor necrosis factor alpha, whereas all VDC subsets released comparable levels of IL-10 and IL-2 cytokines. Furthermore, OX62+VDCs from infected rats primed naïve CD4+T-cell proliferation and release of cytokines, including gamma interferon, IL-2, IL-6, and IL-10, in response to staphylococcal enterotoxin B stimulation in vitro. Adoptive transfer of highly purified OX62+VDCs from infected rats induced a significant acceleration of fungal clearance compared with that in rats receiving naive VDCs, suggesting a protective role of VDCs in the anti-Candidamucosal immunity. Finally, VDC-mediated protection was associated with their ability to rapidly migrate to the vaginal mucosa and lymph nodes, as assessed by adoptive transfer of OX62+VDCs labeled with 5 (and 6-)-carboxyfluorescein diacetate succinimidyl ester. |
| Databáze: | OpenAIRE |
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