Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-Reperfusion

Autor: Bing-Mei Zhu, Wan-xin Liu, Fanrong Liang, Yan Huang, Wei-Xing Shen, Ning Wang, Qian Li, Su-Yun He, Sheng-Feng Lu, Shu-Ping Fu, Chen-Jun Hu
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Male
medicine.medical_treatment
Ischemia
Acupuncture Therapy
Pharmaceutical Science
Gene Expression
Myocardial Reperfusion Injury
Pharmacology
medicine.disease_cause
Article
Analytical Chemistry
lcsh:QD241-441
Electrocardiography
lcsh:Organic chemistry
Drug Discovery
Gene expression
medicine
gene expression profiling
Animals
Cluster Analysis
Physical and Theoretical Chemistry
Receptor
Regulation of gene expression
business.industry
pathway
Organic Chemistry
Reproducibility of Results
medicine.disease
Rats
Gene expression profiling
Disease Models
Animal
Cytokine
Gene Expression Regulation
myocardial ischemia-reperfusion
Chemistry (miscellaneous)
electro-acupuncture pretreatment
Immunology
Molecular Medicine
RNA-seq
Vascular smooth muscle contraction
business
Oxidative stress
Genome-Wide Association Study
Signal Transduction
Zdroj: Molecules
Volume 19
Issue 10
Pages 16158-16178
Molecules, Vol 19, Iss 10, Pp 16158-16178 (2014)
ISSN: 1420-3049
DOI: 10.3390/molecules191016158
Popis: This study investigated genome-wide gene expressions and the cardioprotective effects of electro-acupuncture pretreatment at the PC6 Neiguan acupoint on myocardial ischemia reperfusion (I/R) injury. Male SD rats were randomly divided into four groups: sham operation (SO), I/R, electro-acupuncture at the PC6 Neiguan acupoint pretreatment (EA) and electro-acupuncture at non-acupoint pretreatment (NA). Compared with the I/R group, the survival rate of the EA group was significantly increased, the arrhythmia score, infarction area, serum concentrations of CK, LDH and CK-Mb and plasma level of cTnT were significantly decreased. RNA-seq results showed that 725 genes were up-regulated and 861 genes were down-regulated under I/R conditions compared to the SO group
both EA and NA reversed some of these gene expression levels (592 in EA and 238 in NA group). KEGG pathway analysis indicated that these genes were involved in multiple pathways, including ECM, MAPK signaling, apoptosis, cytokine and leukocyte pathways. In addition, some pathways were uniquely regulated by EA, but not NA pretreatment, such as oxidative stress, cardiac muscle contraction, gap junction, vascular smooth muscle contraction, hypertrophic, NOD-like receptor, and P53 and B-cell receptor pathways. This study was first to reveal the gene expression signatures of acute myocardial I/R injury and electro-acupuncture pretreatment in rats.
Databáze: OpenAIRE
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