Epigenetic Aberrations Are Not Specific to Transcription Factor-Mediated Reprogramming

Autor: Adele Gabriele Marthaler, Hans R. Schöler, Ulf Tiemann, Natalia Tapia, Guangming Wu
Rok vydání: 2016
Předmět:
Pluripotent Stem Cells
0301 basic medicine
Cellular differentiation
Induced Pluripotent Stem Cells
Kruppel-Like Transcription Factors
Biology
Biochemistry
Germline
Cell Line
Epigenesis
Genetic
Kruppel-Like Factor 4
Mice
03 medical and health sciences
Report
Genetics
Animals
Humans
Epigenetics
Induced pluripotent stem cell
lcsh:QH301-705.5
Cells
Cultured
Homeodomain Proteins
lcsh:R5-920
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Cell Differentiation
Nanog Homeobox Protein
Cell Biology
DNA Methylation
Cellular Reprogramming
Cell biology
Germ Cells
030104 developmental biology
lcsh:Biology (General)
DNA methylation
Somatic cell nuclear transfer
Stem cell
lcsh:Medicine (General)
Octamer Transcription Factor-3
Reprogramming
Transcription Factors
Developmental Biology
Zdroj: Stem Cell Reports
Stem Cell Reports, Vol 6, Iss 1, Pp 35-43 (2016)
ISSN: 2213-6711
Popis: Summary Somatic cells can be reprogrammed to pluripotency using different methods. In comparison with pluripotent cells obtained through somatic nuclear transfer, induced pluripotent stem cells (iPSCs) exhibit a higher number of epigenetic errors. Furthermore, most of these abnormalities have been described to be intrinsic to the iPSC technology. Here, we investigate whether the aberrant epigenetic patterns detected in iPSCs are specific to transcription factor-mediated reprogramming. We used germline stem cells (GSCs), which are the only adult cell type that can be converted into pluripotent cells (gPSCs) under defined culture conditions, and compared GSC-derived iPSCs and gPSCs at the transcriptional and epigenetic level. Our results show that both reprogramming methods generate indistinguishable states of pluripotency. GSC-derived iPSCs and gPSCs retained similar levels of donor cell-type memory and exhibited comparable numbers of reprogramming errors. Therefore, our study demonstrates that the epigenetic abnormalities detected in iPSCs are not specific to transcription factor-mediated reprogramming.
Highlights • GSCs can be converted into iPSCs and into gPSCs under specific culture conditions • iPSCs and gPSCs retain the same level of donor cell-type epigenetic memory • Comparable numbers of reprogramming errors can be detected in iPSCs and gPSCs • Epigenetic aberrations are not specific to transcription factor-mediated reprogramming
Tapia, Schöler, and colleagues converted germline stem cells into pluripotent iPSCs using transcription factors and into pluripotent gPSCs using specific culture conditions. They detected similar levels of donor cell-type memory and reprogramming errors in both pluripotent cell types. These results demonstrate that epigenetic aberrations are not specific to transcription factor-mediated reprogramming.
Databáze: OpenAIRE
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