Efficacy of systemic administration of riboflavin on a rabbit model of corneal alkali burn
Autor: | Ekaterina Lobashova, Olga Żuk, Maksym Żuk, Sławomir Wierzba |
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Rok vydání: | 2020 |
Předmět: |
Male
medicine.medical_specialty genetic structures Riboflavin Femoral vein lcsh:Medicine Alkalies Administration Cutaneous Injections Intramuscular 030226 pharmacology & pharmacy Article Cornea 03 medical and health sciences Route of administration Medical research 0302 clinical medicine Ciliary body Ophthalmology Burns Chemical medicine Animals Humans lcsh:Science Multidisciplinary business.industry Aqueous humour lcsh:R eye diseases Sclera Disease Models Animal Eye Burns medicine.anatomical_structure Injections Intra-Arterial Outcomes research 030221 ophthalmology & optometry Systemic administration lcsh:Q Female Rabbits sense organs Choroid business Corneal Injuries |
Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-7 (2020) |
ISSN: | 2045-2322 |
Popis: | Changes in the barrier mechanisms in the eye should determine the rational route for the administration and dosage of each drug in the treatment of traumatic injuries and other pathologies. The aim of this study was to examine the efficacy of intra-arterial delivery of 14C-riboflavin (as an “indicator”) and compare it with intravenous and intramuscular administration in an animal model of chemical eye burn. 14C-riboflavin (14C-I) was administered by intra-arterial (carotid artery), intravenous (femoral vein) and intramuscular (femoral muscle) routes. The total radioactivity was determined over 2 h in the plasma and structures of the rabbit’s eyes using a scintillation counter. The results of the study show that intravascular administration of 14C-I gives significantly higher concentrations of total radioactivity in the blood and is accompanied by a significant increase in the permeability of the blood-barrier and barrier in eyes suffering from burns. The highest concentration in the plasma and aqueous humour of the anterior chamber of the eye was observed during the first hour with the intra-arterial route of administration of 14C-I in either burnt and unburnt eyes. The distribution of total radioactivity in the structures of the eye over the 2 h of the experiment showed a higher level of the drug under intra-arterial administered in the uveal regions, namely: the iris, ciliary body, choroid, retina and also the sclera and cornea. This experimental model shows that intra-arterial administration can increase the bioavailability of a drug to the structures of the eye within a short period of time. |
Databáze: | OpenAIRE |
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